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Analysis of the Specificity of Auto-Reactive Antibodies to Individual Fragments of the Extracellular Domain of Desmoglein 3 in Patients with Pemphigus Vulgaris

  • EXPERIMENTAL METHODS FOR CLINICAL PRACTICE
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Bulletin of Experimental Biology and Medicine Aims and scope

A method for the analysis of the epitope specificity of auto-reactive antibodies to desmoglein 3 (Dsg3) using competitive ELISA has been developed. It is based on a two-stage solid-phase ELISA with initial “depletion” of auto-reactive antibodies against the studied epitope and subsequent quantitative assessment of antibodies against full-length extracellular domain Dsg3. The proposed approach for assessing the specificity of the autoimmune response in patients with pemphigus vulgaris can provide in the future the possibility to personalize the therapy using plasmapheresis by preliminary selection of the antigenic composition of the extracorporeal immunosorbent.

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Authors and Affiliations

  1. State Scientific Center for Dermatovenereology and Cosmetology, Ministry of Health of the Russian Federation, Moscow, Russia

    A. A. Kubanov, D. G. Deryabin, M. V. Shpilevaya, A. E. Karamova & A. A. Nikonorov

  2. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia

    E. N. Larina, D. A. Dolgikh, T. V. Bobik, I. V. Smirnov, A. G. Gabibov & M. P. Kirpichnikov

  3. M. V. Lomonosov Moscow State University, Moscow, Russia

    T. K. Aliev, D. A. Dolgikh & M. P. Kirpichnikov

Authors
  1. A. A. Kubanov
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  2. D. G. Deryabin
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  3. M. V. Shpilevaya
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  4. A. E. Karamova
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  5. A. A. Nikonorov
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  6. E. N. Larina
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  7. T. K. Aliev
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  8. D. A. Dolgikh
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  9. T. V. Bobik
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  10. I. V. Smirnov
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  11. A. G. Gabibov
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  12. M. P. Kirpichnikov
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Corresponding author

Correspondence to A. A. Kubanov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 4, pp. 490-495, April, 2021

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Kubanov, A.A., Deryabin, D.G., Shpilevaya, M.V. et al. Analysis of the Specificity of Auto-Reactive Antibodies to Individual Fragments of the Extracellular Domain of Desmoglein 3 in Patients with Pemphigus Vulgaris. Bull Exp Biol Med 171, 475–479 (2021). https://doi.org/10.1007/s10517-021-05254-9

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  • DOI: https://doi.org/10.1007/s10517-021-05254-9

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