Changes in cerebral neurogenesis provoked by ischemia and the effect of fluoxetine on this process were studied using a three-vessel occlusion model of global transient cerebral ischemia. The global transient cerebral ischemia was modeled on male Wistar rats by transient occlusion of three major vessels originating from the aortic arch and supplying the brain (brachiocephalic trunk, left subclavian artery, and left common carotid artery). The cells expressing doublecortin (DCX, a marker of young neurons) were counted in the hippocampal dentate gyrus on day 31 after ischemia modeling. It was found that ischemia inhibited neurogenesis in the dentate gyrus in comparison with sham-operated controls (p<0.05), while fluoxetine (20 mg/kg/day) injected over 10 days after surgery restored neurogenesis to the control level (p<0.001).
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References
Cayre M, Canoll P, Goldman JE. Cell migration in the normal and pathological postnatal mammalian brain. Prog. Neurobiol. 2009; 88(1):41-63.
Chernysheva GA, Smol’yakova VI, Osipenko AN, Plotnikov MB. Evaluation of survival and neurological deficit in rats in the new model of global transient cerebral ischemia. Bull. Exp. Biol. Med. 2014; 158(2):97-119.
Gonen M, Panageas KS, Larson SM. Statistical issues in analysis of diagnostic imaging experiments with multiple observations per patient. Radiology. 2001; 221:763-767.
Kee NJ, Preston E, Wojtowicz JM. Enhanced neurogenesis after transient global ischemia in the dentate gyrus of the rat. Exp. Brain Res. 2001; 136:313-320.
Keilhoff G, John R, Langnaese K, Schweizera H, Ebmeyer U. Triggered by asphyxia neurogenesis seems not to be an endogenous repair mechanism, gliogenesis more like it. Neuroscience. 2010; 171:869-884.
Li WL, Cai HH, Wang B, Chen L, Zhou QG, Luo CX, Liu N, Ding XS, Zhu DY. Chronic fluoxetine treatment improves ischemia-induced spatial cognitive deficits through increasing hippocampal neurogenesis after stroke. J. Neurosci. Res. 2009; 87:112-122.
Liu J, Solway K, Messing RO, Sharp FR. Increased neurogenesis in the dentate gyrus after transient global ischemia in gerbils. J. Neurosci. 1998; 18:7768-7778.
Malberg JE, Eisch AJ, Nestler EJ, Duman RS. Chronic antidepressant treatment increases neurogenesis in adult rat hippocampus. J. Neurosci. 2000; 20:9104-9110.
Nakatomi H, Kuriu T, Okabe S, Yamamoto S, Hatano O, Kawahara N, Tamura A, Kirino T, Nakafuku M. Regeneration of hippocampal pyramidal neurons after ischemic brain injury by recruitment of endogenous neural progenitors. Cell. 2002; 110:429-441.
Pforte C, Henrich-Noack P, Baldaufa K, Reymann KG. Increase in proliferation and gliogenesis but decrease of early neurogenesis in the rat forebrain shortly after transient global ischemia. Neuroscience. 2005; 136:1133-1146.
Salazar-Colocho P, Lanciego JL, Del Rio J, Frechilla D. Ischemia induces cell proliferation and neurogenesis in the gerbil hippocampus in response to neuronal death. Neurosci. Res. 2008; 61:27-37.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 3, pp. 325-329, March, 2016
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Khodanovich, M.Y., Kisel’, A.A., Chernysheva, G.A. et al. Effect of Fluoxetine on Neurogenesis in Hippocampal Dentate Gyrus after Global Transient Cerebral Ischemia in Rats. Bull Exp Biol Med 161, 351–354 (2016). https://doi.org/10.1007/s10517-016-3412-4
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DOI: https://doi.org/10.1007/s10517-016-3412-4