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3D-Technology of the Formation and Maintenance of Single Dormant Microspheres from 2000 Human Somatic Cells and Their Reactivation In Vitro

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Bulletin of Experimental Biology and Medicine Aims and scope

We developed an original reproducible 3D-technology for preparation of single dormant microspheres consisting of 2000 somatic cells. The dynamics of microsphere assembly from mesenchymal and epithelial cells of retinal pigment epithelium was traced using time-lapse microscopy: formation of a loose aggregate over 24 h followed by its gradual consolidation and formation of a compact viable microsphere with a diameter of 100–150 μ by day 7. The cell number in the formed microspheres remains unchanged. Reactivation observed upon fusion of epithelial and/or mesenchymal microspheres results in the formation of a united compact microtissue. The fusion dynamics reproduces spherogenesis irrespective of the initial amount of co-cultured microspheres. Reactivation via two-step induced angiogenesis opens new prospects for production of vascularized microspheres and microtissues.

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Authors and Affiliations

  1. Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia

    V. S. Repin, I. N. Saburina, N. V. Kosheleva, A. A. Gorkun, I. M. Zurina & A. A. Kubatiev

  2. Russian Medical Academy of Postgraduate Education, Ministry of Health of the Russian Federation, Moscow, Russia

    V. S. Repin, I. N. Saburina & A. A. Kubatiev

  3. Biological Faculty of M. V. Lomonosov Moscow State University, Moscow, Russia

    N. V. Kosheleva

Authors
  1. V. S. Repin
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  2. I. N. Saburina
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  3. N. V. Kosheleva
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  4. A. A. Gorkun
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  5. I. M. Zurina
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  6. A. A. Kubatiev
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Corresponding author

Correspondence to N. V. Kosheleva.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 161–168, July, 2014

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Repin, V.S., Saburina, I.N., Kosheleva, N.V. et al. 3D-Technology of the Formation and Maintenance of Single Dormant Microspheres from 2000 Human Somatic Cells and Their Reactivation In Vitro . Bull Exp Biol Med 158, 137–144 (2014). https://doi.org/10.1007/s10517-014-2709-4

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  • DOI: https://doi.org/10.1007/s10517-014-2709-4

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