Mini-sequencing with subsequent result registration using MALDI-ToF mass-spectrometry was employed for hepatitis B virus genetic typing in Russian population. This approach was employed for hepatitis B virus genetic typing in HBsAg-positive patients with chronic hepatitis B, hepatitis of combined etiology and hepatic cirrhosis and allowed to show the prevalence of D genotype (83.3%) in all groups of patients. Other hepatitis B virus genotypes: genotype A (5.9%), genotype C (3.6%), and mixed infection with D and C (7.2%) were also found in patients with chronic hepatitis B and hepatic cirrhosis. All genotypes were found in patients with chronic hepatitis B and hepatic cirrhosis. Chronic hepatitis of combined etiology was noted only in patients with genotype D. Possibility of detection of mixed infection with hepatitis B viruses of various genotypes is a distinct advantage of mini-sequencing approach over direct nucleotide sequence evaluation for hepatitis B virus genetic typing.
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References
E. N. Ilina, M. V. Malakhova, E. V. Generozov, et al., Biomed. Chim., 51, No. 1, 41-47 (2005).
P. Araus-Ruiz, H. Norder, K. A. Visona, and L. O. Magnius, J. Med. Virol., 51, No. 4, 305-312 (1997).
H. L. Chan, M. Hussain, and A. S. Loc, Hepatology, 29, No. 3, 976-984 (1999).
G. Francois, M. Kew, P. Van Damme, et al., Vaccine, 19, No. 28-29, 3799-3815 (2001).
D. Holmes and M. Quigley, Anal. Biochem., 114, No. 1, 193-197 (1981).
H. L. Janssen, M. van Zonneveld, H. Senturk, et al., Lancet., 365, 123-129 (2005).
J. H. Kao, P. J. Chen, M. Y. Lai, and D. S. Chen, Gastroenterology, 118, No. 3, 554-559 (2000).
M. Lindh, J. E. Gonzalez, G. Norkrans, and P. Horal, J. Virol. Method., 72, No. 2, 163-174 (1998).
C. M. Lo, C. K. Cheung, G. K. Lau, et al., Am. J. Transplant., 5, No. 8, 1893-1900 (2005).
V. M. Morozov, M. M. Pisareva, and M. P. Groudinin, Gene, 260, Nos. 1-2, 55-65 (2000).
J. Sambrook, E. F. Fritsch, and T. Maniatis, Molecular cloning: a laboratory manual, N. Y. (1989).
J. M. Sanchez-Tapias, J. Costa, A. Mas, et al., Gastroenterology, 123, No. 6, 1848-1856 (2002).
L. Stuyver, S. De Gendt, C. Van Geyt, et al., J. Gen. Virol., 81, Pt. 1, 67-74 (2000).
S. Payungporn, P. Tangkijvanich, P. Jantaradsamee, et al., J. Virol. Methods, 120, No. 2, 131-140 (2004).
Y. Song, E. Dai, J. Wang, et al., Mol. Cell. Probes, 20, No. 2, 121-127 (2006).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 147, No. 2, pp. 181-186, February, 2009
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Malakhova, M.V., Ilina, E.N., Govorun, V.M. et al. Hepatitis B Virus Genetic Typing Using Mass-Spectrometry. Bull Exp Biol Med 147, 220–225 (2009). https://doi.org/10.1007/s10517-009-0479-1
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DOI: https://doi.org/10.1007/s10517-009-0479-1