Abstract
Binding of zinc cations to human serum γ-globulin was studied by molecular ultrafiltration. The content of free metal in the filtrate was evaluated by reaction with o-phenanthroline. Conformation characteristics of the protein were determined by UV spectrophotometry. Our findings suggest that γ-globulin molecule contains several zinc binding sites differing by corresponding constants and successively occupied with increase in the content of bound metal. The parameters of zinc binding correspond to those obtained in experiments with copper. Conformation status of protein with bound zinc differs significantly from that of protein with bound copper cations.
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E. E. Babaeva, U. A. Vorobyova, M. S. Zharkova, and S. B. Cheknyov, Byull. Eksp. Biol. Med., 141, No. 1, 59–62 (2005).
M. V. Vol'kenstein, Mol. Biol., 25, No. 4, 883–925 (1991).
S. M. Kiselyov, S. V. Lutsenko, S. E. Severin, and E. S. Severin, Biokhimiya, 68, No. 5, 611–631 (2003).
V. I. Lim, Biofizika, 36, No. 3, 441–454 (1991).
S. B. Cheknyov, E. E. Babaeva, U. A. Vorobyova, and E. A. Denisova, Med. Immunol., 7, No. 4, 375–380 (2005).
S. B. Cheknyov, E. E. Babaeva, U. A. Vorobyova, and E. A. Denisova, Byull. Eksp. Biol. Med., 140, No. 8, 177–180 (2005).
D. B. Borza and W. T. Morgan, J. Biol. Chem., 273, No. 10, 5493–5499 (1998).
C. Driessen, K. Hirv, H. Kirchner, and L. Rink, Immunology, 84, No. 2, 272–277 (1995).
J. D. Fraser, R. G. Urban, J. L. Strominger, and H. Robinson, Proc. Natl. Acad. Sci. USA, 89, No. 12, 5507–5511 (1992).
N. N. Gorgani, C. R. Parish, and J. G. Altin, J. Biol. Chem., 274, No. 42, 29,633–29,640 (1999).
M. Oblender and U. Carpentieri, J. Cancer Res. Clin. Oncol., 117, No. 5, 444–448 (1991).
L. Regan and N. D. Clarke, Biochemistry, 29, No. 49, 10,878–10,883 (1990).
L. Rink and H. Kirchner, J. Nutr., 130, Suppl. 5, S1407–S1411 (2000).
V. A. Roberts, B. L. Iverson, S. A. Iverson, et al., Proc. Natl. Acad. Sci. USA, 87, No. 17, 6654–6658 (1990).
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Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 141, No. 5, pp. 540–543, May, 2006
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Babaeva, E.E., Vorobyova, U.A., Denisova, E.A. et al. Binding of zinc cations to human serum γ-globulin. Bull Exp Biol Med 141, 602–605 (2006). https://doi.org/10.1007/s10517-006-0232-y
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DOI: https://doi.org/10.1007/s10517-006-0232-y