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Yeast cell walls stimulate viability, respiratory burst, and phagocytosis in channel catfish (Ictalurus punctatus) head-kidney macrophages

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Abstract

Yeast cell wall (YCW) preparations are important nutritional additives in the aquaculture industry. Head-kidney macrophages play important roles in innate immune functions in fish. However, the mechanisms underlying their stimulation are not yet characterized. The purpose of this study was to investigate the effects of YCW on immunological functions of channel catfish (Ictalurus punctatus) head-kidney macrophages. Our results showed that YCW preparations not only improved the proliferation and phagocytosis of macrophages but also induced the secretion of reactive oxygen species (ROS), nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin 1β (IL-1β). At a concentration of 500.0 μg/mL, YCW preparations stimulated macrophage proliferation and phagocytosis to 1.98 and 1.8 times, respectively, when compared with the negative group. Equally, YCW preparations raised the production of NO by inducing iNOS and ROS in a concentration-dependent manner. Furthermore, these preparations enhanced TNF-α and IL-1β mRNA levels to multiple digits from 1.2 to 3.0 times, respectively. In conclusion, the stimulatory effects of YCW on the phagocytic activity of the fish macrophages were associated with respiratory bursts coupled with nitric oxide production.

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Acknowledgments

We thank the International Science Editing (http://www.internationalscienceediting.com) for editing this manuscript.

Funding

This work was funded by the Science and Technology Project of Hubei Province Department of Education (B2018076), the Open Research Project from Key Laboratory of Fishery Drug Development Ministry of Agriculture (201505), the Introduction Talent Research Start-up Fund of Wuhan Polytechnic University (2016284), and the Wuhan Polytechnic University Scientific Research Project (201740).

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Authors and Affiliations

  1. Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, School of Animal Sciences and Nutritional Engineering, Wuhan Polytechnic University, Wuhan City, 430023, Hubei Province, China

    Jianjun Zhou, Zhen Gao, Wenbiao Wang, Feng Huang, Rui Wang, Wumin Yang, Xianqin Hu & Xuedong Wang

  2. Angel Yeast Co., Ltd., Yichang City, 443003, Hubei Province, China

    Junpeng Hu & Aqiong Gong

  3. Agricultural Comprehensive Service Station of Dong Xihu Farm Administration of Hubei Province, Wuhan City, 430023, Hubei Province, China

    Jie Li

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  1. Jianjun Zhou
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  2. Zhen Gao
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  3. Wenbiao Wang
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  4. Feng Huang
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  5. Junpeng Hu
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  7. Rui Wang
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  9. Jie Li
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  10. Xianqin Hu
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  11. Xuedong Wang
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Correspondence to Xianqin Hu or Xuedong Wang.

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Zhou, J., Gao, Z., Wang, W. et al. Yeast cell walls stimulate viability, respiratory burst, and phagocytosis in channel catfish (Ictalurus punctatus) head-kidney macrophages. Aquacult Int 27, 1655–1665 (2019). https://doi.org/10.1007/s10499-019-00417-y

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  • DOI: https://doi.org/10.1007/s10499-019-00417-y

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