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Genetic diversity of freshwater pearl mussel (Hyriopsis cumingii) in populations from the five largest lakes in China revealed by inter-simple sequence repeat (ISSR)

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Abstract

Triangle sail mussel (Hyriopsis cumingii) is the most important mussel species commercially exploited for freshwater pearl culture in China. Its genetic diversity was studied among populations from the five largest freshwater lakes of China, Poyang Lake (PY), Dongting Lake (DT), Taihu Lake (TH), Hongze Lake (HZ), and Chaohu Lake (CH), by the polymorphism of the inter-simple sequence repeats (ISSRs). Polymerase chain reaction (PCR) detections showed that a total of 62 loci were amplified from eight primers; of those, 31.88%, 31.15%, 31.03%, 27.27% and 26.92%, respectively, were polymorphic in each of the five populations across all genotypes tested. The average heterozygosities were 0.4747, 0.3274, 0.2366, 0.2099 and 0.2018 in each of these populations from PY, TH, DT, CH and HZ, respectively. Phylogenic analyses showed that PY and TH populations clustered with CH and HZ and formed a group, while the DT population on its own formed a separated branch. The smallest distance (0.0404) was scored between PY and TH populations, indicative of their closest relationship; the biggest distance (0.2438) was found between PY and DT populations, suggesting their greatest divergence. The present study provided genetic basis for managing mussel stocks from these lakes separately to best preserve the genetic diversity of H. cumingii. On the other hand, since the population in PY displayed the highest genetic diversity, it may be used preferably in future selective breeding to improve pearl yield and quality.

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Abbreviations

PY:

Poyang Lake

DT:

Dongting Lake

TH:

Taihu Lake

HZ:

Hongze Lake

CH:

Chaohu Lake

ISSR:

Inter-simple sequence repeat

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Acknowledgements

We wish to thank Qiqun Cheng of East China Sea Fisheries Research Institute for his assistance in the preparation of the manuscript. This work was supported by grants from the Basic Research Priorities Program of the Science (06DJ14003) and Shanghai Leading Academic Discipline Project (Y1101).

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  1. College of Aqua-Life Science and Technology, Shanghai Fisheries University, 334, Jungong Road, Shanghai, 200090, China

    J. L. Li, G. L. Wang & Z. Y. Bai

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  1. J. L. Li
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  2. G. L. Wang
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  3. Z. Y. Bai
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Correspondence to J. L. Li.

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Li, J.L., Wang, G.L. & Bai, Z.Y. Genetic diversity of freshwater pearl mussel (Hyriopsis cumingii) in populations from the five largest lakes in China revealed by inter-simple sequence repeat (ISSR). Aquacult Int 17, 323–330 (2009). https://doi.org/10.1007/s10499-008-9204-8

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