Abstract
Temperature is an important factor influencing the physiological activities of agricultural pests. Therefore, understanding pest physiological activities and the molecular response to high-temperature stress is of paramount importance for pest management. Tetranychus truncatus Ehara (Acari: Tetranychidae) is a harmful organism, that may cause serious harm to crops such as corn and cotton in high-temperature environments. Cytochrome P450 (CYP450) is induced by high-temperature stress, and it plays an important role in the resistance of spider mites to high temperatures. Because of their role in high-temperature tolerance, the cytochrome P450 genes of the spider mite are attracting more and more attention. In this study, we identified and analyzed CYP450 genes in T. truncatus to investigate their potential roles in growth and development and the resistance to high-temperature stress. Based on phylogenetic and structural analyses, we identified 17 CYP450 genes in T. truncatus. RNA-seq and real-time quantitative PCR (RT-qPCR) revealed differential expression patterns of these genes at different developmental stages and levels of high-temperature stress resistance. The RNA interference results of selected CYP450 showed that when TtCYP3A2 and TtCYP4V2 were silenced by feeding on dsRNA, respectively, the high-temperature resistance of T. truncatus was decreased, which indicated that the expression of these two CYP450 genes in this species may be related to high-temperature tolerance. Our results provide potential evidence for the response of spider mites to high-temperature stress and help to improve the understanding of T. truncatus’s ability to resist high-temperature stress.
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Acknowledgements
We are grateful to Liwen Song PhD for her valuable comments on an earlier version and would like to thank all the participants and appreciate very much the valuable comments and suggestions by the reviewers.
Funding
This research was funded by the National Natural Science Funds of China (No. 31860503). Key team construction project of Agricultural insect and pest control in Gansu Agricultural University (GSAU-XKJS-2023).
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All authors contributed to the study conception and design, material preparation, data collection and analyses. The first draft of the manuscript was written by LW and all authors commented on subsequent versions of the manuscript.
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Li, W., Wu, X., Hu, T. et al. The role of cytochrome P450 3A2 and 4V2 in response to high-temperature stress in Tetranychus truncatus (Acari: Tetranychidae). Exp Appl Acarol 91, 263–277 (2023). https://doi.org/10.1007/s10493-023-00837-9
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DOI: https://doi.org/10.1007/s10493-023-00837-9