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Oxidation of biological molecules with age and induced oxidative stress in different growth phases of Saccharomyces cerevisiae

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Abstract

One of the mechanistic approaches for explaining ageing is the oxidative stress theory of ageing. Saccharomyces cerevisiae has been used as a model to study ageing due to many factors. We have attempted to investigate if the differential ability to withstand oxidative stress can be correlated with their lifespans. In all the four strains studied (AP22, 699, 8C, and SP4), there was no age-associated increases in lipid peroxidation levels measured as thiobarbituric acid reactive substances (TBARS). Under induced oxidative stress conditions, there was an increased TBARS level in both the ages assessed with a quantum-fold increase in the stationary phase cells of AP22. In contrast, the late stationary phase cells of 8C exhibited the least susceptibility to induced oxidative stress. The level of TBARS in both exponential and late stationary phase cells of 699 was overall more than that in the other three strains. Protein carbonylation increased with age in 8C and 699. Induced stress increased carbonylation in the exponential cells of SP4 and 699 and the stationary phase cells of all four strains. Protein carbonylation data indicate that the AP22 cells exhibit decreased protein carbonylation vis-à-vis the other strains. Induced stress data showed that while the exponential cells of 699 are susceptible, the late stationary phase cells of 699 are most resistant. Western blotting analysis using anti-HNE antibodies showed two proteins of molecular mass ~ 56 and ~ 84 kDa that were selectively modified with age in all the strains. Under induced stress conditions, an additional protein of ~ 69 kDa was oxidized. Our investigation shows that the difference in lifespan between the four strains of S. cerevisiae may be regulated by oxidative stress. Knowledge of the identity of the oxidized proteins will significantly facilitate a better understanding of the effect of oxidative stress conditions on the cells of S. cerevisiae.

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Acknowledgements

The authors thank Prof. T. Choudhuri, Department of Biotechnology, for the use of the Bio-Rad ChemiDoc™ XRS+ gel imaging system and Dr. Surjya Sakia, Department of Zoology, for help with the statistical analysis. The authors thank Dr. Kalpana Bhargava, DRDO, for the silver staining protocol. Assistance from Ms. Ankita Nandi for the preparation of the figures is also acknowledged.

Funding

This study was funded by grants from Science and Engineering Research Board/DST, India (SR/FT/LS-103/2008). MM thanks University Grants Commission, India for non-NET fellowship.

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  1. Madhumathan Mukherjee

    Present address: St. Teresa School, Santiniketan, Dist. Birbhum, 731235, India

Authors and Affiliations

  1. Department of Biotechnology, Visva-Bharati University, Santiniketan, West Bengal, 731235, India

    Madhumathan Mukherjee & Nilanjana Das

  2. Department of Chemistry, Purash-Kanpur Haridas Nandi Mahavidyalaya, P.O. Kanpur, Howrah, West Bengal, 711410, India

    Chandan Kumar Jana

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MM performed the experiments, CKJ was involved in designing the experiments, data analysis and editing of the manuscript; ND was involved in designing the experiments, data analysis and writing and editing the manuscript.

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Correspondence to Nilanjana Das.

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Mukherjee, M., Jana, C.K. & Das, N. Oxidation of biological molecules with age and induced oxidative stress in different growth phases of Saccharomyces cerevisiae. Antonie van Leeuwenhoek 116, 353–365 (2023). https://doi.org/10.1007/s10482-022-01807-8

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