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Rouxiella aceris sp. nov., isolated from tree sap and the emended description of the genus Rouxiella

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A Correction to this article was published on 25 August 2021

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Abstract

Polyphasic taxonomic studies were performed for the seven strains, which were isolated from sap extracted from Acer pictum in Mt. Halla in Jeju, Republic of Korea. Cells of all the isolates were Gram-reaction-negative, facultatively anaerobic, short rods and contained the major isoprenoid quinone of Q-8, the predominant fatty acids of C16:0 and C17:0 cyclo and the major polar lipids including phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid. The G + C contents of the genomic DNAs were 50.6–51.3%.The 16S rRNA gene-based phylogeny exhibited that the seven isolates formed two distinct sublines within the family Yersiniaceae. In the 92 core gene analysis, strain SAP-1T formed a subline at the base of radiation of the genus Rouxiella and its assignment to the genus Rouxiella was supported by high amino acid identity values (82.0–83.4%), albeit with sharing low 16S rRNA gene identities (96.0–96.9%). The average nucleotide identity and digital DNA-DNA hybridisation values together with phenotypic differences showed that strains SAP-1T, SAP-7, SAP-8 and SAP-13 belonged to a new species of the genus Rouxiella, while strains SAP-2, SAP-3 and SAP-27 were strains of Rouxiella silvae. On the basis of data obtained here, Rouxiella aceris sp. nov. (type strain, SAP-1T = KCTC 72599T = CCM 9078T) is proposed, with the emended description of the genus Rouxiella.

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Funding

This research was carried out in part by a research grant supported from the Ministry of Food and Drug Safety, Korea (Grant No. MFDS2019-69).

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Authors and Affiliations

  1. Institute of Jeju Microbial Resources, BioPS Co., Ltd, Jeju, 63243, Republic of Korea

    Soon Dong Lee

  2. Institute of Hallasan Ecology and Culture, Jeju, 63064, Republic of Korea

    Hong Lim Yang

  3. Department of Biological Sciences and Biotechnology, Hannam University, Daejon, 34054, Republic of Korea

    In Seop Kim

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  1. Soon Dong Lee
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  2. Hong Lim Yang
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Contributions

Bacterial isolation from tree sap was performed by Hong-Lim Yang. Data collection and analysis were performed by Soon Dong Lee and Hong-Lim Yang. The first draft of the manuscript was written by Soon Dong Lee. All authors read and approved the final manuscript.

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Correspondence to Soon Dong Lee.

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Lee, S.D., Yang, H.L. & Kim, I.S. Rouxiella aceris sp. nov., isolated from tree sap and the emended description of the genus Rouxiella. Antonie van Leeuwenhoek 114, 1013–1024 (2021). https://doi.org/10.1007/s10482-021-01572-0

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