Abstract
Bacteria need to adopt to different behavioral tuning depending on the dynamic eco-physiological conditions they are exposed to. One of these adaptive strategies is the use of motility. Here we report the twitching motility response of four endophytic isolates of Bacillus sp. when exposed to different eco-physiological stimuli like different nutrient sources, and mechanical and chemical antagonists on solid surfaces. These endophytic bacteria were isolated from different parts of a hemiparasite Loranthus sp. Jacq. (Loranthaceae) growing on economically important mango trees. The results show that the twitching motility of these bacteria was more when exposed to organic acids, metals salts (among nutrients) and mechanical shearing (stress) than the other factors. Their motility is not affected by surface lubrication or EPS production, but instead is influenced by shear-sensitive structures and affinity to metal ions. Further molecular studies are needed to elucidate the basis of this twitching behaviour on solid surfaces.
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Acknowledgements
The authors are grateful to Dr. Mohabul Alam Mandal, Department of Chemistry, University of Gour Banga, W.B., India for spectroscopic analysis, and Dr. Prajnamita Dasgpta and Sri Soumyadeep Laha, University Science Instrumentation Centre, University of North Bengal, W.B., India for SEM study. We are also grateful to Dr. Sanjukta Mondal Parui, Associate Professor and HOD, Department of Zoology, Lady Brabourne College, W.B., India for her help in language editing and Samrat Hore, Assistant Professor, Department of Statistics, Tripura University, Tripura, India for his help in validating the statistical analysis.
Funding
V. Mandal is grateful to the Department of Science and Technology, Science and Engineering Board, Government of India for funding the project (DST-SERB/F/5369/2013-’14; dated 19.11.2013) for partial financial help of the work.
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RA, SK, PK Maiti and PK Mitra did the experimental work and prepared the manuscript and SM and VM designed the work plan, and manuscript editing.
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The GenBank Accession number of Bacillus sp. LSB-01 is MF039483; Bacillus sp. LSB-02 is MF037703; Bacillus sp. LSB-03 is MF037704; and Bacillus sp. LLB-04 is MF037706.
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Adhikary, R., Kundu, S., Maiti, P.K. et al. Effect of different stimuli on twitching behavior of endophytic bacteria isolated from Loranthus sp. Jacq.. Antonie van Leeuwenhoek 113, 1489–1505 (2020). https://doi.org/10.1007/s10482-020-01458-7
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DOI: https://doi.org/10.1007/s10482-020-01458-7