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Ottowia flava sp. nov., isolated from fish intestines

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Abstract

A novel Gram-negative bacterium, non-motile and short rod-shaped, designated strain GY511T, was isolated from the intestines of fish collected from Maowei Sea, China. Growth occurred at pH 6.0–9.0 (optimum 7.0), 4–37 °C (optimum 28 °C) and at 0–2.5% (w/v) NaCl (optimum 1.0%). The result of 16S rRNA gene sequence analysis showed that strain GY511T is closely related to O. oryzae NBRC 113109T (97.6%), O. konkukae DSM 105395T (97.4%), Ottowia beijingensis CGMCC 1.12324T (95.9%), Ottowia pentelensis DSM 21699T (95.2%) and Ottowia thiooxydans DSM 14619T (95.0%). The DNA–DNA hybridization values of strain GY511T with O. oryzae NBRC 113109T and O. konkukae DSM 105395T were 35.4 ± 3.1% and 26.3 ± 1.8%, respectively. The major fatty acids (> 10%) were identified as summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16:0 and summed feature 8 (C18:1ω7c and/or C18:1ω6c) and the major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, two unidentified aminolipids and an unidentified phospholipid. The G+C content of the genomic DNA was 62.9 mol%. Thiosulfate could be utilized as co-substrate for aerobic growth and was oxidised to sulfate. On the basis of phenotypic, chemotaxonomic and molecular data, strain GY511T is considered to represent a novel species of the genus Ottowia, for which the name Ottowia flava sp. nov. is proposed. The type strain is GY511T (= NBRC 113500T = DSM 107425T = CGMCC 1.13650T).

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Acknowledgements

This research was funded by National Natural Science Foundation of China (Nos. 31460005 and 31660005) and supported by Major research Project of Guangxi for science and technology (AA18242026), Guangxi Natural Science Foundation Project (2016GXNSFAA380075), Scientific and technological breakthrough in Nanning (20153330), Science and technology base and talent special in Guangxi (AD18126005), Xiangsihu Young Scholars Innovative Research Team of Guangxi University for Nationalities (2017) and Innovation Project of Guangxi Graduate Education (No.gxun-chxzs 2017123).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning, 530008, People’s Republic of China

    Song-Biao Shi, Li-Fang Yang & Cong Liu

  2. Guangxi Key Laboratory of Utilization of Microbial and Botanical Resources, College of Marine Biotechnology, Guangxi University for Nationalities, Nanning, 530008, People’s Republic of China

    Ming-Guo Jiang, Jia-Fa Wu & Nai-Kun Shen

  3. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Song-Biao Shi, Gui-Ding Li, Qin-Yuan Li, Kun Zhang, Long-Qian Jiang, Cheng-Lin Jiang & Yi Jiang

  4. Institute of Microbial Pharmaceuticals, Northeastern University, Shenyang, 110819, People’s Republic of China

    Gui-Ding Li

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Contributions

S-BS: performed research and wrote the paper; G-DL: analyzed data; L-FY: guide experiment; CL; Collection of samples; M-GJ: designed study; Q-YL: analyzed data; J-FW: collection of samples and performed research; KZ: performed research; L-QJ: performed research; N-KS: collection of samples; C-LJ: designed study; YJ: designed study and wrote the paper.

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Correspondence to Ming-Guo Jiang or Yi Jiang.

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Shi, SB., Li, GD., Yang, LF. et al. Ottowia flava sp. nov., isolated from fish intestines. Antonie van Leeuwenhoek 112, 1567–1575 (2019). https://doi.org/10.1007/s10482-019-01284-6

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