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Promicromonospora viridis sp. nov., a novel actinomycete isolated from soil

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Abstract

Two Gram-stain positive, aerobic actinomycete strains, designated NEAU-JGR1T and NEAU-JGC41, were isolated from soil collected from Fairy Lake Botanical Garden in Shenzhen, Guangdong Province, south of China. The 16S rRNA gene sequences analysis showed that the two strains exhibited 99.5% 16S rRNA gene sequence similarity with each other and were closely related to Promicromonospora thailandica JCM 17130T (99.4, 99.3%) and Promicromonospora citrea DSM 43110T (99.2, 99.2%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains clustered together and formed a cluster with P. thailandica JCM 17130T and P. citrea DSM 43110T. Both strains were observed to contain MK-9(H4) and MK-9(H2) as predominant menaquinones. Their whole cell sugar profiles were found to main contained rhamnose, ribose, glucose and galactose. The phospholipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycophosphatidylinositol, phosphatidylinositol mannoside, an unidentified glycolipid and an unidentified phospholipid. The predominant cellular fatty acids for the two strains were identified as anteiso-C15:0, iso-C15:0 and anteiso-C17:0. The DNA–DNA hybridization value between strains NEAU-JGR1T and NEAU-JGC41 was 85.1 ± 0.3%, and the values between the two strains and their close phylogenetic relatives were well below 70%, supporting the conclusion that they represent a distinct genomic species. An array of phenotypic characteristics also differentiated the isolates from closely related species. On the basis of the genetic and phenotypic properties, strains NEAU-JGR1T and NEAU-JGC41 can be classified as representatives of a novel species of the genus Promicromonospora, for which the name Promicromonospora viridis sp. nov., is proposed. The type strain is NEAU-JGR1T (= DSM 105536T = CGMCC 4.7473T).

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Acknowledgements

This work was supported in part by grants from the National key research and development plan (No. 2017YFD0201606) and the National Natural Science Foundation of China (Nos. 31572070 and 31672092), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP). We thank Prof. Aharon Oren (The Hebrew University of Jerusalem) for helping to derive the name viridis for the two strains.

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Liying Jin, Junwei Zhao, Shanwen Jiang, Yang Zhao, Xiaoyang Han, Xiaowei Guo, Xiangjing Wang & Wensheng Xiang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Wensheng Xiang

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  1. Liying Jin
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  2. Junwei Zhao
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  3. Shanwen Jiang
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  4. Yang Zhao
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Jin, L., Zhao, J., Jiang, S. et al. Promicromonospora viridis sp. nov., a novel actinomycete isolated from soil. Antonie van Leeuwenhoek 111, 2079–2086 (2018). https://doi.org/10.1007/s10482-018-1099-4

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