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Transformation of the yeast Trichosporonoides oedocephalis

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Abstract

The osmotolerant yeast, Trichosporonoides oedocephalis, is an excellent producer of erythritol, which has wide industrial applications. In this study, we developed an efficient transformation method for T. oedocephalis. To evaluate the T. oedocephalis transformation, we constructed a DNA fragment (loxP-Kan-loxP/Cre system) that was targeted to the mitogen-activated protein kinase HOG1 gene. Transformants were selected on plates containing G418 and response surface methodology was employed to obtain optimum transformation conditions. Optimal transformation could be achieved at an incubation time of 40 min, when the concentration of zymolyase-100T was 30 µg/mL, and when 100 mM CaCl2 was added to the mixture. The predicted optimal transformation efficiency was 133 transformants per µg of DNA. This novel method will facilitate studies in synthetic biology and metabolic engineering of T. oedocephalis.

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Acknowledgments

This study was supported in part by the National Natural Science Foundation of China (Grant No: 21376156) and by the agricultural infrastructure project of Suzhou Science and Technology Development Plan (Grant No: SYN201321 and SYN201412).

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Authors and Affiliations

  1. School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou, 215009, People’s Republic of China

    Liangzhi Li, Tianyi Yang, Chao Hu, Xin Ju, Cuiying Hu & Bingyu Tang

  2. Key Laboratory of New Energy and Low-carbon Technology of Suzhou City, Suzhou University of Science and Technology, Suzhou, 215009, People’s Republic of China

    Liangzhi Li

  3. School of Materials, University of Manchester, Manchester, M13 9PL, UK

    Chao Hu

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  1. Liangzhi Li
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  2. Tianyi Yang
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  3. Chao Hu
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  4. Xin Ju
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  5. Cuiying Hu
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  6. Bingyu Tang
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Correspondence to Liangzhi Li.

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Li, L., Yang, T., Hu, C. et al. Transformation of the yeast Trichosporonoides oedocephalis . Antonie van Leeuwenhoek 109, 305–309 (2016). https://doi.org/10.1007/s10482-015-0633-x

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