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Meiothermus roseus sp. nov., a thermophilic bacterium isolated from a geothermal area

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Abstract

Two closely related thermophilic bacterial strains, designated YIM 71031T and YIM 71039, were isolated from a hot spring in Tengchong county, Yunnan province, south-western China. The novel isolates were observed to be Gram-negative, aerobic, rod-shaped and yellow-pigmented bacteria. The strains were found to be able to grow at 37–65 °C, pH 6.0–9.0 and with a NaCl tolerance up to 1.0 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequences placed these two isolates in the genus Meiothermus. They were found to be closely related to Meiothermus timidus DSM 17022T (98.6 % similarity), and formed a cluster with this species. The predominant menaquinone was identified as MK-8 and the major fatty acids (>10 %) as anteiso-C15:0, iso-C15:0, anteiso-C17:0, iso-C16:0 and C16:0. The genomic DNA G+C contents of strains YIM 71031T and YIM 71039 were determined to be 64.0 and 65.4 mol%, respectively. DNA–DNA hybridizations showed low values between strains YIM 71031T and YIM 71039 and their closely related neighbour M. timidus DSM 17022T. Morphological phylogenetic and chemotaxonomic results suggest that strains YIM 71031T and YIM 71039 are representatives of a new species within the genus Meiothermus, for which the name Meiothermus roseus sp. nov. is proposed. The type strain is YIM 71031T (=KCTC 42495T =NBRC 110900T).

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Acknowledgments

The authors are grateful to Prof. Hans-Peter Klenk (DSMZ, Germany) for his kind providing of the reference type strain. This research was supported by the Key Project of International Cooperation of Ministry of Science & Technology (MOST) (No. 2013DFA31980), Natural Science Foundation of China (Nos. 31372545 and 31470139), Program for Innovative Research Team (in Science and Technology) in University of Henan Province (14IRTSTHN013), Plan for Scientific Innovation Talent of Henan Province (154100510010), Scientific Research Fund of Xinxiang Medical University (2013QN126), Research Project of Education Department of Henan Province of China (2011A180025). W.-J. Li was also supported by the Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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    Authors and Affiliations

    1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

      Hong Ming, Yi-Rui Yin, En-Min Zhou, Lan Liu & Wen-Jun Li

    2. College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

      Hong Ming

    3. College of Fisheries, Henan Normal University, Xinxiang, 453007, People’s Republic of China

      Yan-Yan Duan, Qian-Qian Guo, Shuai Li & Guo-Xing Nie

    4. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

      Wen-Jun Li

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    Hong Ming and Yan-Yan Duan contributed equally to this study.

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    Ming, H., Duan, YY., Guo, QQ. et al. Meiothermus roseus sp. nov., a thermophilic bacterium isolated from a geothermal area. Antonie van Leeuwenhoek 108, 897–905 (2015). https://doi.org/10.1007/s10482-015-0544-x

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