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Fontibacillus solani sp. nov. isolated from potato (Solanum tuberosum L.) root

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Abstract

A bacterial strain designated A4STR04T was isolated from the inner root tissue of potatoes in Spain. Phylogenetic analysis based on the 16S rRNA gene sequence placed the isolate into the genus Fontibacillus, being most closely related to Fontibacillus panacisegetis KCTC 13564T with 99 % identity. The isolate was observed to form Gram-positive, motile and sporulating rods. The catalase test was found to be negative and oxidase positive. Nitrate was found to be reduced to nitrite. β-Galactosidase and caseinase were observed to be produced but the production of gelatinase, urease, arginine dehydrolase, ornithine and lysine decarboxylase was negative. Aesculin hydrolysis was found to be positive and acetoin production was negative. Growth was found to be supported by many carbohydrates and organic acids as carbon source. MK-7 was the only menaquinone detected and the major fatty acid (61.5 %) was identified as anteiso-C15:0, as occurs in the other species of genus Fontibacillus. The strain A4STR04T was found to display a complex lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a glycolipid, two phospholipids, a lipid and two aminophospholipids. Mesodiaminopimelic acid was detected in the peptidoglycan. The G+C content was determined to be 50.5 mol% (Tm). Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain A4STR04T (=LMG 28458 T = CECT 8693T) should be classified as representing a novel species of genus Fontibacillus, for which the name Fontibacillus solani sp. nov. is proposed.

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Acknowledgments

This research was funded by Junta de Castilla y León (Spanish Regional Government) under project CSI327A12-2. MHRB is recipient of a JAE-Doc researcher contract from CSIC cofinanced by ERDF. We are grateful to IDEBIO S. L. and V. Souza-Egipsy from ICA Electron Microscopy Service.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

This article does not contain any studies with human participants or animals performed by any of the authors.

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Authors and Affiliations

  1. Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), c/Cordel de Merinas 40-52, 37008, Salamanca, Spain

    Martha-Helena Ramírez-Bahena, Maria José Cuesta, Jose Mariano Igual & Alvaro Peix

  2. Unidad Asociada Grupo de Interacción Planta-Microorganismo, Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain

    Martha-Helena Ramírez-Bahena, Jose Mariano Igual, Encarna Velázquez & Alvaro Peix

  3. Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain

    José David Flores-Félix, Carmen Tejedor Gil & Encarna Velázquez

  4. Centro Regional de Diagnóstico, Junta de Castilla y León, Salamanca, Spain

    Jose Luis Palomo & Pablo García Benavides

  5. Instituto de Ciencias Agrarias ICA-CSIC, Madrid, Spain

    Mercedes Fernández Pascual

Authors
  1. Martha-Helena Ramírez-Bahena
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  2. José David Flores-Félix
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  3. Maria José Cuesta
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  4. Carmen Tejedor Gil
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  5. Jose Luis Palomo
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  6. Pablo García Benavides
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  7. Jose Mariano Igual
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  8. Mercedes Fernández Pascual
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  9. Encarna Velázquez
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  10. Alvaro Peix
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Corresponding author

Correspondence to Alvaro Peix.

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Ramírez-Bahena, MH., Flores-Félix, J.D., Cuesta, M.J. et al. Fontibacillus solani sp. nov. isolated from potato (Solanum tuberosum L.) root. Antonie van Leeuwenhoek 107, 1315–1321 (2015). https://doi.org/10.1007/s10482-015-0426-2

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  • DOI: https://doi.org/10.1007/s10482-015-0426-2

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