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Tropicihabitans flavus gen. nov., sp. nov., a new member of the family Cellulomonadaceae

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An Erratum to this article was published on 14 May 2015

Abstract

Two novel Gram-stain positive actinobacteria, designated PS-14-16T and RS-7-1, were isolated from the rhizosphere of a mangrove and sea sediment, respectively, and their taxonomic positions were investigated by a polyphasic approach. Both strains were observed to form vegetative hyphae in the early phase of growth but the hyphae eventually fragment into short rods to coccoid cells. The peptidoglycan type of both strains was found to be A4α. Their predominant menaquinone was identified as MK-9(H4) and the major fatty acid as anteiso-C15:0. The DNA G+C content was determined to be 68.4–68.5 mol%. 16S rRNA gene sequencing revealed that strains PS-14-16T and RS-7-1 were related to members of the family Cellulomonadaceae. Their nearest phylogenetic neighbour was found to be Sediminihabitans luteus, which is currently the only species of the genus Sediminihabitans, with a similarity of 97.94 %. However, strains PS-14-16T and RS-7-1 were distinguishable from the members of the genus Sediminihabitans and the other genera within the family Cellulomonadaceae in terms of chemotaxonomic characteristics and phylogenetic relationship. The results of DNA–DNA hybridization experiments indicated that strains PS-14-16T and RS-7-1 belong to the same species. Strains PS-14-16T and RS-7-1 are concluded to represent a novel genus and species of the family Cellulomonadaceae, for which the name Tropicihabitans flavus gen. nov., sp. nov. is proposed. The type strain of T. flavus is PS-14-16T (=NBRC 110109T = IanCC A 516T).

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Acknowledgments

This work was supported by Science and Technology Research Partnership for Sustainable Development (SATREPS) which is a research program in collaborated with the Japan Science and Technology Agency (JST) and the Japan International Cooperation Agency (JICA).

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Authors and Affiliations

  1. Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan

    Moriyuki Hamada, Chiyo Shibata, Tomohiko Tamura & Ken-ichiro Suzuki

  2. Indonesian Institute of Sciences (LIPI), Jl, Raya Jakarta-Bogor Km. 46, Cibinong, 16911, Indonesia

    Arif Nurkanto, Shanti Ratnakomala & Puspita Lisdiyanti

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  1. Moriyuki Hamada
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  2. Chiyo Shibata
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  6. Tomohiko Tamura
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  7. Ken-ichiro Suzuki
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Correspondence to Moriyuki Hamada.

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Hamada, M., Shibata, C., Nurkanto, A. et al. Tropicihabitans flavus gen. nov., sp. nov., a new member of the family Cellulomonadaceae . Antonie van Leeuwenhoek 107, 1299–1306 (2015). https://doi.org/10.1007/s10482-015-0424-4

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