Abstract
A novel Gram-negative, non-spore forming, rod-shaped aerobic bacterium, designated SSW136T, was isolated from a surface seawater sample collected at Espalamaca (in Faial Island), Azores. Growth was found to occur from 10 to 37 °C, pH 6.0–8.0, and with 2–11 % of NaCl. 16S rRNA gene sequence indicated that the strain SSW136T belongs to the family Rhodobacteraceae. Strain SSW136T exhibited 96.3, 95.9, 95.7 and 95.5 sequence similarity to the type strains Oceanicola litoreus M-M22T, Roseovarius aestuarii SMK-122T, Marivita geojedonensis DPG-138T, and Pseudoruegeria aquimaris SW-255T respectively. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSW136T was affiliated to the family Rhodobacteraceae and formed a separate branch. The G+C content was 63.5 mol%. The major respiratory quinone was found to be Q-10. The polar lipids of strain SSW136T consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and three unidentified phospholipids. The major fatty acids were C18:1 ω7c (46.5 %), Cyclo-C19:0 ω8c (16.0 %) and C16:0 (12.8 %). On the basis of the morphological, genotypic, chemotaxonomic characteristics and low DNA–DNA relatedness, strain SSW136T is proposed to represent a novel genus and novel species, Nioella nitratireducens gen. nov., sp. nov., in the family Rhodobacteraceae. The type strain is SSW136T (=KCTC 32417T = NCIM 5499T).
Similar content being viewed by others
References
Cerny G (1978) Studies on aminopeptidase for the distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:113–122
Collee JG, Fraser AG, Marmion BP, Simmons A (2008) Mackie and McCartney practical medical microbiology, 14th edn. Elsevier Private Limited, Gurgaon
Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470
Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229
Farmer JJ, Hickman-Brenner FW (2006) The genera Vibrio and Photobacterium. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The Prokaryotes, a handbook on the biology of bacteria, 3rd edn. Springer, New York, pp 508–563
Felsenstein J (1985) Conference limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789
Fitch WM (1971) Toward defining the course of evolution: minimum change for specified tree topology. Syst Zool 20:406–416
Garrity GM, Bell JA, Lilburn T (2005) Family I. Rhodobacteraceae fam. nov. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s Manual of Systematic Bacteriology, 2nd edn, vol. 2 (The Proteobacteria), part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria). Springer, New York, p 161
Khandeparker R, Verma P, Meena RM, Deobagkar DD (2011) Phylogenetic diversity of carbohydrate degrading culturable bacteria from Mandovi and Zuari estuaries, Goa, west coast of India. Estuar Coast Shelf Sci 95:359–366
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematic. Wiley, New York, pp 115–175
Lee SY, Kang CH, Oh TK, Yoon JH (2012) Virgibacillus campisalis sp. nov., from a marine solar saltern. Int J Syst Evol Microbiol 62:347–351
Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of isoprenoid quinines and polar lipids. J Microbiol Methods 2:233–241
Park S, Lee MH, Yoon JH (2013) Oceanicola litoreus sp. nov., an alphaproteobacterium isolated from the seashore sediment. Antonie Van Leeuwenhoek 103:859–866
Romanenko LA, Tanaka N, Frolova GM, Svetashev VI, Mikhailov VV (2011) Litoreibacter albidus gen. nov., sp. nov. and Litoreibacter janthinus sp. nov., members of the class Alphaproteobacteria isolated from the seashore. Int J Syst Evol Microbiol 61:148–154
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc., Newark, DE
Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Wang H, Zhang X, Yan S, Qi Z, Yu Y, Zhang X-H (2012) Huaishuia halophila gen. nov., sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 62:223–228
Yoon J-H, Kang S-J, Lee J-S (2013) Marivita geojedonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63:423–427
Yoon J-H, Kang S-J, Oh T-K (2008) Roseovarius aestuarii sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 58:1198–1202
Yoon J-H, Lee S-Y, Kang S-J, Lee C-H, Oh T-K (2007) Pseudoruegeria aquimaris gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:542–547
Acknowledgments
The authors are grateful to Dr. N. Ramaiah and R. M. Meena for providing DNA sequence facility. Author Rajasabapathy acknowledges CSIR for providing senior research fellowship. We acknowledge Department of Science & Technology, Govt. of India for the support through an Indo-Portugal bilateral program. We acknowledge MMRF-COMAPS under ICMAM, MoES for FAME facility supported by Dr. Shanta Nair, Dr. Anas Abdulaziz and V. Vijitha. We thank Dr. V. K. Banakar and Mr. Sarath for SEM analysis. IMAR-DOP/UAz is Research and Development Unit #531 and LARSyS—Associated Laboratory # 9 funded by the Portuguese Foundation for Science and Technology (FCT) through PEst project (Pest/OE/EEI/LA0009/2011–2014), and by DRCTC—Regional Government of the Azores through a Pluriannual Funding scheme. This is CSIR-NIO contribution number 5686.
Author information
Authors and Affiliations
Corresponding author
Additional information
The GenBank/EMBL/DDBJ accession number of the 16S rRNA gene sequence of the strain SSW136T is KC534331.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Rajasabapathy, R., Mohandass, C., Yoon, JH. et al. Nioella nitratireducens gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island. Antonie van Leeuwenhoek 107, 589–595 (2015). https://doi.org/10.1007/s10482-014-0355-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-014-0355-5