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Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil

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Abstract

An alkalitolerant actinomycete strain, designated B32T, was isolated from a Saharan soil sample collected from Adrar province (South of Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce short chains of spores on the dichotomous branched aerial mycelium and formed a fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 0–5 % (w/v) and the optimum growth temperature and pH were found to be 25–35 °C and 7.0–10.0 °C, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain B32T were identified as MK-10 (H4) and MK-11 (H4). The major fatty acids were found to be iso-C16:0 and anteiso-C15:0. The diagnostic phospholipids detected were phosphatidylcholine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The chemotaxonomic properties of strain B32T are consistent with those shared by members of the genus Nocardiopsis. 16S rRNA gene sequence analysis indicated that strain B32T is most closely related to Nocardiopsis alba DSM 43377T (98.7 %), Nocardiopsis lucentensis DSM 44048T (98.6 %), Nocardiopsis aegyptia DSM 44442T (98.6 %), Nocardiopsis sinuspersici HM6T (98.6 %) and Nocardiopsis arvandica HM7T (98.5 %). However, the DNA–DNA relatedness values between strain B32T and the closely related type strains were 17.9, 14.6, 31.1, 27.1 and 14.1 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain B32T should be classified as representative of a novel species, for which the name Nocardiopsis algeriensis sp. nov. is proposed. The type strain is B32T (=DSM 45462T = CECT 8712T).

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Acknowledgments

We would like to gratefully acknowledge the technical assistance of Gabriele Pötter and Bettina Sträubler (both at DSMZ).

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Authors and Affiliations

  1. Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria

    Noureddine Bouras, Atika Meklat, Abdelghani Zitouni & Nasserdine Sabaou

  2. Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, 47000, Ghardaïa, Algeria

    Noureddine Bouras

  3. Département de Biologie et physiologie cellulaire, Faculté des Sciences de la Nature et de la Vie, Université Saâd Dahleb, Blida, Algeria

    Atika Meklat

  4. Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique, UMR 5503 (CNRS/INPT/UPS), 1 Avenue de l’Agrobiopôle BP 32607 Auzeville Tolosane, 31326, Castanet-Tolosan, France

    Florence Mathieu

  5. Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany

    Peter Schumann, Cathrin Spröer & Hans-Peter Klenk

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  1. Noureddine Bouras
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  2. Atika Meklat
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  3. Abdelghani Zitouni
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  7. Nasserdine Sabaou
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Correspondence to Nasserdine Sabaou or Hans-Peter Klenk.

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Bouras, N., Meklat, A., Zitouni, A. et al. Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil. Antonie van Leeuwenhoek 107, 313–320 (2015). https://doi.org/10.1007/s10482-014-0329-7

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  • DOI: https://doi.org/10.1007/s10482-014-0329-7

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