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Paenibacillus yonginensis sp. nov., a potential plant growth promoting bacterium isolated from humus soil of Yongin forest

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Abstract

Strain DCY84T, a Gram-stain positive, rod-shaped, aerobic, spore-forming bacterium, motile by means of peritrichous flagella, was isolated from humus soil from Yongin forest in Gyeonggi province, South Korea. Strain DCY84T shared the highest sequence similarity with Paenibacillus barengoltzii KACC 15270T (96.86 %), followed by Paenibacillus timonensis KACC 11491T (96.49 %) and Paenibacillus phoenicis NBRC 106274T (95.77 %). Strain DCY84T was found to able to grow best in TSA at temperature 30 °C, at pH 8 and at 0.5 % NaCl. MK-7 menaquinone was identified as the isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified aminolipids and an unidentified polar lipid. The peptidoglycan was found to contain the amino acids meso-diaminopimelic acid, alanine and d-glutamic acid. The major fatty acids of strain DCY84T were identified as branched chain anteiso-C15:0, saturated C16:0 and branched chain anteiso-C17:0. The cell wall sugars of strain DCY84T were found to comprise of ribose, galactose and xylose. The major polyamine was identified as spermidine. The DNA G+C content was determined to be 62.6 mol%. After 6 days of incubation, strain DCY84T produced 52.96 ± 1.85 and 72.83 ± 2.86 µg/ml l-indole-3-acetic acid, using media without l-tryptophan and supplemented with l-tryptophan, respectively. Strain DCY84T was also found to be able to solubilize phosphate and produce siderophores. On the basis of the phenotypic characteristics, genotypic analysis and chemotaxonomic characteristics, strain DCY84T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus yonginensis sp. nov. is proposed. The type strain is DCY84T (=KCTC 33428T = JCM 19885T).

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Acknowledgments

This research was supported by Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries (KIPET NO: 309019-03-3-SB010) and Next-Generation BioGreen 21 Program (SSAC, grant#: PJ009529032014), Republic of Korea.

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Authors and Affiliations

  1. Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si, 449-701, Gyeonggi-do, Republic of Korea

    Johan Sukweenadhi, Yeon-Ju Kim, Kwang Je Lee, Van-An Hoang, Ngoc-Lan Nguyen & Deok-Chun Yang

  2. Department of Environmental Engineering, Korea Maritime University, Busan, Republic of Korea

    Sung-Cheol Koh

  3. Faculty of Biotechnology, University of Surabaya (UBAYA), Surabaya, Indonesia

    Johan Sukweenadhi

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  1. Johan Sukweenadhi
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Correspondence to Yeon-Ju Kim or Deok-Chun Yang.

Electronic supplementary material

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10482_2014_263_MOESM1_ESM.tif

The maximum likelihood tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships of strain DCY84T and members of the genus Paenibacillus. Supplementary material 1 (TIFF 12396 kb)

10482_2014_263_MOESM2_ESM.tif

Transmission electron micrograph of Paenibacillus yonginensis DCY84T. Bar indicated 1.0 μm. Supplementary material 2 (TIFF 2889 kb)

10482_2014_263_MOESM3_ESM.tif

Plant growth promoting activity test: a Clear halo regions observed around the colonies on opaque Pikovskaya medium indicate the positive result for the phosphate solubilization test. b Siderophore production test using Pseudomonas Agar F medium (Difco) supplemented with a chrome azurol S complex [CAS/iron(III)/hexadeciltrimethyl ammonium bromide]. The yellow halo zones on blue-green colour medium indicates apositive result for siderophores production by strain DCY84T. Supplementary material 3 (TIFF 5984 kb)

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Sukweenadhi, J., Kim, YJ., Lee, K.J. et al. Paenibacillus yonginensis sp. nov., a potential plant growth promoting bacterium isolated from humus soil of Yongin forest. Antonie van Leeuwenhoek 106, 935–945 (2014). https://doi.org/10.1007/s10482-014-0263-8

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