Abstract
A novel Gram-stain-positive, motile, hemolytic, endospore-forming and rod-shaped bacterium was isolated and designated as strain GIESS003T. The strain grew optimally at 35 °C, at pH 7.0–7.5, and with 3.0–3.5 % (w/v) NaCl. The 16S rRNA gene sequence analysis indicated that strain GIESS003T was associated with the genus Ornithinibacillus and was most closely related to the type strain of Ornithinibacillus contaminans (96.5 % similarity). The major cellular fatty acids were iso-C15:0 and anteiso-C15:0. The polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was menaquinone-7. Strain GIESS003T contained a peptidoglycan of type A4β l-Orn-d-Asp. The G+C content of genomic DNA was 40.1 mol%. On the basis of polyphasic evidence from this study, a new species of the genus Ornithinibacillus, Ornithinibacillus heyuanensis sp. nov., is proposed, with strain GIESS003T (=KCTC 33159T=CCTCC 2013106T) as the type strain.
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Acknowledgments
This work was supported by the Team Project of Guangdong Natural Science Foundation (S2012030006144), the Science and Technology Planning Project of Guangdong Province, China (2012B091100242 and 2012B030800008) and Guangzhou Science and Technology Development Foundation, China (2011J2200039).
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Chu Wu and Ming Chang have contributed equally to this work.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA of strain GIESS003T is KF317693.
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Wu, C., Chang, M., Yang, G. et al. Ornithinibacillus heyuanensis sp. nov., isolated from South China. Antonie van Leeuwenhoek 106, 235–241 (2014). https://doi.org/10.1007/s10482-014-0186-4
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DOI: https://doi.org/10.1007/s10482-014-0186-4