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Halolamina rubra sp. nov., a haloarchaeon isolated from non-purified solar salt

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An Erratum to this article was published on 26 June 2015

Abstract

Two Gram-stain negative, rod-shaped and motile extreme halophiles, designated CBA1107T and CBA1108, were isolated from non-purified solar salt. Based on the phylogenetic analysis, strains CBA1107T and CBA1108 were shown to belong to the genus Halolamina, with similarities for the 16S rRNA gene sequences between strains CBA1107T and Halolamina pelagica TBN21T , Halolamina salina WSY15-H3T and Halolamina salifodinae WSY15-H1T of 98.3, 97.6 and 97.3 %, respectively; the similarities for the rpoB′ gene sequences between the same strains were 96.0, 95.3 and 94.6 %, respectively. The colonies of both strains were observed to be red pigmented on growth medium. Strain CBA1107T was observed to grow at 20–50 °C, in the presence of 15–30 % NaCl, at pH 6.0–9.0, and with 0.005–0.5 M Mg2+. The cells of both strains lysed in distilled water. The DNA–DNA hybridization experiments showed that strain CBA1107T shared 97 % relatedness with CBA1108 and <50 % relatedness with H. pelagica JCM 16809T, H. salina JCM 18549T and H. salifodinae JCM 18548T. The genomic DNA G+C content of strain CBA1107T was determined to be 65.1 mol%. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and glycolipids including sulfated mannosyl glucosyl diether and mannosyl glucosyl diether. Based on the polyphasic taxonomic analyses, the strains are considered to represent a new taxon for which the name Halolamina rubra sp. nov. is proposed, with the type strain CBA1107T (=CECT 8421T =JCM 19436T).

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2012R1A1A2040922) and a project fund (C33730) to J. S. Choi from the Center for Analytical Research of Disaster Science at the Korea Basic Science Institute.

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Authors and Affiliations

  1. Jeju Center, Korea Basic Science Institute, Jeju, 690-140, Republic of Korea

    In-Tae Cha, Kyung June Yim, Hye Seon Song, Hae-Won Lee, Kil-Nam Kim, Daekyung Kim & Seong Woon Roh

  2. Department of Microbiology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    In-Tae Cha & Sung-Keun Rhee

  3. World Institute of Kimchi, Gwangju, 503-360, Republic of Korea

    Hae-Won Lee & Hak-Jong Choi

  4. Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Dong-Wook Hyun, Sung-Jae Lee & Jin-Woo Bae

  5. Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806, Republic of Korea

    Jong-Soon Choi

  6. Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea

    Jong-Soon Choi

  7. Division of Bioengineering, Incheon National University, Incheon, 406-772, Republic of Korea

    Myung-Ji Seo

  8. Fermentation and Functionality Research Group, Korea Food Research Institute, Sungnam, 463-746, Republic of Korea

    Young-Do Nam

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  1. In-Tae Cha
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Correspondence to Seong Woon Roh or Young-Do Nam.

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Cha, IT., Yim, K.J., Song, H.S. et al. Halolamina rubra sp. nov., a haloarchaeon isolated from non-purified solar salt. Antonie van Leeuwenhoek 105, 907–914 (2014). https://doi.org/10.1007/s10482-014-0145-0

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