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Roseivivax atlanticus sp. nov., isolated from surface seawater of the Atlantic Ocean

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Abstract

A taxonomic study was carried out on strain 22II-S10sT, which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, oxidase and catalase positive, rod shaped and motile by subpolar flagella. The isolate was capable of gelatine hydrolysis but unable to reduce nitrate to nitrite or degrade Tween 80 or aesculin. Growth was observed at salinities of 0.5–18 % (optimum, 2–12 %), at pH of 3–10 (optimum, 7) and at temperatures of 10–41 °C (optimum 28 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S10sT belongs to the genus Roseivivax, with highest sequence similarity to Roseivivax halodurans JCM 10272T (97.2 %), followed by Roseivivax isoporae LMG 25204T (97.0 %); other species of genus Roseivivax shared 95.2–96.7 % sequence similarity. The DNA–DNA hybridization estimate values between strain 22II-S10sT and the two type strains (R. halodurans JCM 10272T and R. isoporae LMG 25204T) were 22.00 and 21.40 %. The principal fatty acids were identified as Summed Feature 8 (C18:1 ω7c/ω6c) (67.4 %), C18:0 (7.2 %), C19:0 cyclo ω8c (7.1 %), C18:1 ω7c 11-methyl (6.8 %) and C16:0 (5.9 %). The respiratory quinone was determined to be Q-10 (100 %). Phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, a glycolipid and three phospholipids were present. The G+C content of the chromosomal DNA was determined to be 67.5 mol%. The combined genotypic and phenotypic data show that strain 22II-S10sT represents a novel species within the genus Roseivivax, for which the name Roseivivax atlanticus sp. nov. is proposed, with the type strain 22II-S10sT (= MCCC 1A09150T = LMG 27156T).

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Abbreviations

MCCC:

Marine Culture Collection of China

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Acknowledgments

This work was financially supported by COMRA program (No. DY125-15-R-01), National Natural Science Foundation of China (41276005/41176154/40906083), Public Welfare Project of SOA (201005032) and National Infrastructure of Natural Resources for Science and Technology Program of China (No. NIMR-2013-9 and NIMR-2014-9).

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Authors and Affiliations

  1. State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Key Laboratory of Marine Genetic Resources of Fujian Province, Collaborative Innovation Center of Deep Sea Biology, Xiamen, 361005, China

    Guizhen Li, Qiliang Lai, Xiupian Liu, Fengqin Sun & Zongze Shao

  2. Life Science College, Xiamen University, Xiamen, 361005, China

    Guizhen Li & Qiliang Lai

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  1. Guizhen Li
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  2. Qiliang Lai
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  3. Xiupian Liu
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  4. Fengqin Sun
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  5. Zongze Shao
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Correspondence to Zongze Shao.

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Guizhen Li and Qiliang Lai contributed equally to this work.

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Li, G., Lai, Q., Liu, X. et al. Roseivivax atlanticus sp. nov., isolated from surface seawater of the Atlantic Ocean. Antonie van Leeuwenhoek 105, 863–869 (2014). https://doi.org/10.1007/s10482-014-0140-5

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