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Saccharopolyspora cavernae sp. nov., a novel actinomycete isolated from the Swallow Cave in Yunnan, south-west China

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Abstract

A Gram-positive, aerobic, non-motile actinobacterial strain, designated YIM C01235T, was isolated from a soil sample collected from the Swallow Cave, Yunnan province, south-west China. The isolate grew at 10–30 °C, pH 6.0–9.0 and 0–8 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed highest similarity to Saccharopolyspora gloriosae YIM 60513T (96.8 %), and lower 16S rRNA gene sequence similarities (95.1–96.7 %) with the other species of the genus Saccharopolyspora. The whole-cell hydrolysates contained meso-diaminopimelic acid (meso-DAP), arabinose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unknown glycolipids, two unknown phospholipids and one polar lipid. MK-9(H4) was the predominant menaquinone. The major fatty acids were iso-C15:0, iso-C16:0, anteiso-C15:0 and anteiso-C17:0. Mycolic acids were not detected. The genomic DNA G+C content was 69.1 mol%. These chemotaxonomic data, together with its morphological properties, were consistent with the assignment of strain YIM C01235T to the genus Saccharopolyspora. The results of physiological and biochemical tests allowed strain YIM C01235T to be differentiated phenotypically from all the recognized Saccharopolyspora species. On the basis of evidence from this polyphasic study, the novel species Saccharopolyspora cavernae sp. nov. is proposed. The type strain is YIM C01235T (=DSM 45825T = CCTCC AA 2012022T).

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Acknowledgments

This work was funded jointly by National New Drug Research and Development Project (No. 2010ZX09401-403) and project of China tobacco Yunnan industrial Co. Lid. (Nos. 2012JC07 and 2012FL02). W-J Li was supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Juan Cheng, Dao-Feng Zhang & Wen-Jun Li

  2. Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People’s Republic of China

    Juan Cheng, Yong-Guang Zhang, Li Li, Hong-Fei Wang & Wen-Jun Li

  3. Hongyun Honghe Tobacco (Group) Co., Ltd, Kunming, 650202, People’s Republic of China

    Wei Chen & Yan-Qing Duan

  4. The National Engineering Center for Research of Microbial Pharmaceuticals and New Drug Research and Development Center, North China Pharmaceutical Group Corporation, Shijiazhuang, 050015, People’s Republic of China

    Xin-Hua Lu

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  1. Juan Cheng
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  2. Yong-Guang Zhang
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  4. Li Li
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Correspondence to Yan-Qing Duan or Wen-Jun Li.

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Juan Cheng and Yong-Guang Zhang contributed equally to this work.

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Cheng, J., Zhang, YG., Chen, W. et al. Saccharopolyspora cavernae sp. nov., a novel actinomycete isolated from the Swallow Cave in Yunnan, south-west China. Antonie van Leeuwenhoek 104, 837–843 (2013). https://doi.org/10.1007/s10482-013-9996-z

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