Abstract
A novel actinomycete, designated strain NEAU-GH7T, was isolated from a lake sediment and characterized using a polyphasic approach. Strain NEAU-GH7T was Gram-stain positive, aerobic, non-spore-forming and produced spherical sporangia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-GH7T formed a monophyletic clade with the closest relative Streptosporangium longisporum DSM 43180T (99.0 %), an association that was supported by a bootstrap value of 74 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from its closest relative. Moreover, strain NEAU-GH7T could also be differentiated from S. longisporum DSM 43180T and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotypic characteristics, strain NEAU-GH7T should be classified as a new species of the genus Streptosporangium, for which the name Streptosporangium shengliensis sp. nov. is proposed. The type strain is NEAU-GH7T (=CGMCC 4.7105T=DSM 45881T).
Similar content being viewed by others
References
Collins MD (1985) Isoprenoid quinone analyses in bacterial classification and identification. In: Goodfellow M, DE Minnikin (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 267–284
Couch JN (1955) A new genus and family of the Actinomycetales, with a revision of the genus Actinoplanes. J Elisha Mitchell Sci Soc 71:148–155
De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142
Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Gordon RE, Barnett DA, Handerhan JE, Pang C (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63
Hayakawa M, Nonomura H (1987) Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509
Huss VAR, Festl H, Schleifer KH (1983) Studies on the spectrometric determination of DNA hybridisation from renaturation rates. Syst Appl Microbiol 4:184–192
Inahashi Y, Matsumoto A, Ōmura S, Takahashi Yōko (2011) Streptosporangium oxazolinicum sp. nov., a novel endophytic actinomycete producing new antitrypanosomal antibiotics, spoxazomicins. J Antibiot 64:297–302
Kämpfer P, Glaeser SP, Grün-Wollny I, Busse HJ (2013) Streptosporangium sandarakinum sp. nov. Int J Syst Evol Microbiol 63:2484–2489
Kelly KL (1964) Inter-Society Color Council-National Bureau of standards color-name charts illustrated with centroid colors. US Government Printing Office, Washington, DC
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Lechevalier MP, Lechevalier HA (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443
Lechevalier MP, Lechevalier HA (1980) The chemotaxonomy of actinomycetes. In: Dietz A, Thayer DW (eds) Actinomycete taxonomy special publication, vol 6. Society of Industrial Microbiology, Arlington, pp 227–291
Lechevalier MP, De Bievre C, Lechevalier HA (1977) Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:26–249
Lee YK, Kim HW, Liu CL, Lee HK (2003) A simple method for DNA extraction from marine bacteria that produce extracellular materials. J Microbiol Methods 52:245–250
Loqman S, Barka EA, Clément C, Ouhdouch Y (2009) Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold. World J Microbiol Biotechnol 25:81–91
Mandel M, Marmur J (1968) Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
McKerrow J, Vagg S, McKinney T, Seviour EM, Maszenan AM, Brooks P, Se-viour RJ (2000) A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol 30:178–182
Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal K, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
Quintana ET, Goodfellow M (2012) Genus 1. Streptosporangium Couch 1995a, 145AL emend. Stackebrandt Kroppenstedt, Janke, Kemmering and Gűrtler 1994, 268. In: Goodfellow M, Kämpfer P, Busse MJ, Trujillo M, Suzuki KE, Ludwig W, Whitman WB (eds) Bergey’s manual of systematic bacteriology, 2nd edn. Springer, New York
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Sazak A, Sahin N, Camas M, Guven K, Cetin D, Goodfellow M (2012) Streptosporangium anatoliense sp. nov., isolated from soil in Turkey. Antonie Van Leeuwenhoek 102:269–276
Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int Syst Bacteriol 16:313–340
Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) methods for general and molecular bacteriology. American Society for Microbiology, Washington DC, pp 607–654
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Uchida K, Kudo T, Suzuki K, Nakase T (1999) A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56
Waksman SA (1961) The Actinomycetes, vol. 2, classification, identification and descriptions of genera and species. Williams and Wilkins, Baltimore
Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
Wu C, Lu X, Qin M, Wang Y, Ruan J (1989) Analysis of menaquinone compound in microbial cells by HPLC. Microbiology 16:176–178 [English translation of Microbiology (Beijing)]
Xiang WS, Liu CX, Wang XJ, Du J, Xi LJ, Huang Y (2011) Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol 61:1165–1169
Yokota A, Tamura T, Hasegawa T, Huang LH (1993) Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales. Int J Syst Bacteriol 43:805–812
Zhang LP, Jiang CL, Chen WX (2005) Streptosporangium yunnanense sp. nov. and Streptosporangium purpuratum sp. nov., from soil in China. Int J Syst Evol Microbiol 55:719–724
Zhang LP, Zhang LM, Zhang XM (2009) Streptosporangium canum sp. nov., isolated from soil. Int J Syst Evol Microbiol 59:1715–1719
Acknowledgments
This work was supported in part by Grants from the National Key Project for Basic Research (No. 2010CB126102), the National Natural Science Foundation of China (Nos. 30971937 and 30771427), the National Outstanding Youth Foundation (No. 31225024), the Special Foundation for Scientific and Technological Innovation Research of Harbin (No. 2011RFXXN038) and the Natural Science Foundation of Heilongjiang Province (No. C201029).
Author information
Authors and Affiliations
Corresponding authors
Additional information
Xinhui Zhang and Chongxi Liu contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Zhang, X., Liu, C., Zhang, Y. et al. Streptosporangium shengliensis sp. nov., a novel actinomycete isolated from a lake sediment. Antonie van Leeuwenhoek 105, 237–243 (2014). https://doi.org/10.1007/s10482-013-0070-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-013-0070-7