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Streptomyces fukangensis sp. nov., a novel alkaliphilic actinomycete isolated from a saline-alkaline soil

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Abstract

An alkaliphilic actinobacterium, designated EGI 80050T, was isolated from a desert soil sample of Xinjiang, north-west China, and characterized by a polyphasic approach. The isolate was observed to produce purple orange-yellow aerial mycelium and dark orange-yellow substrate mycelium on yeast extract-malt extract agar medium. Whole-cell hydrolysates of strain EGI 80050T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose, rhamnose and mannose as the main sugars. The major fatty acids identified were C16:0-iso (36.8 %), C15:0-anteiso (17.3 %), 15:0-iso (13.2 %) and 14:0-iso (10.5 %). The predominant menaquinones detected were MK-9(H6) and MK-9(H8), while the characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylmethylethanolamine and three unknown phospholipids. The G+C content of the genomic DNA was determined to be 67.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated the strain EGI 80050T to the genus Streptomyces. Levels of 16 rRNA gene sequence similarities between strain EGI 80050T and Streptomyces candidus NRRL ISP-5141T, Streptomyces cremeus NBRC 12760T, Streptomyces spiroverticillatus NBRC 12821T, Streptomyces violaceorectus NBRC 13102T, Streptomyces cinereoruber subsp. cinereoruber NBRC 12756T were 96.7, 96.6, 96.6, 96.6 and 96.6 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain EGI 80050T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces fukangensis sp. nov. (type strain EGI 80050T = BCRC 16945T = JCM 19127T) is proposed.

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Acknowledgments

The authors are grateful to Dr. Hans-Peter Klenk (DSMZ) for providing the reference type strains. This research was supported by the Hundred Talents Program of Chinese Academy of Sciences and the West Light Foundation of Chinese Academy of Sciences (RCPY201203).

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Authors and Affiliations

  1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People’s Republic of China

    Yong-Guang Zhang, Hong-Fei Wang, Yue-Ji Chen, Yuan-Ming Zhang & Wen-Jun Li

  2. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Qing Liu, Juan Cheng & Wen-Jun Li

  3. Bioproducts Research Chair (BRC), College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein, Mohammed A. M. Wadaan & Wen-Jun Li

  4. University of Chinese Academy of Sciences, Beijing, 100049, China

    Hong-Fei Wang

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  1. Yong-Guang Zhang
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Correspondence to Wen-Jun Li.

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Yong-Guang Zhang and Hong-Fei Wang contributed equally to this work.

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Zhang, YG., Wang, HF., Liu, Q. et al. Streptomyces fukangensis sp. nov., a novel alkaliphilic actinomycete isolated from a saline-alkaline soil. Antonie van Leeuwenhoek 104, 1227–1233 (2013). https://doi.org/10.1007/s10482-013-0045-8

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