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Sabulilitoribacter multivorans gen. nov., sp. nov., a polysaccharide-degrading bacterium of the family Flavobacteriaceae isolated from seashore sand

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Abstract

A Gram-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated M-M16T, was isolated from seashore sand around a seaweed farm on the South Sea, South Korea, and its taxonomic position was investigated by using a polyphasic study. Strain M-M16T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Strain M-M16T exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Gaetbulibacter lutimaris (96.5 %) and Flaviramulus basaltis (95.8 %). Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M16T clustered with the type strains of Gaetbulibacter species and F. basaltis. Strain M-M16T contained MK-6 as the predominant menaquinone and iso-C15:1 G, iso-C15:0 and iso-C17:0 3-OH as the major fatty acids. The major polar lipids detected in strain M-M16T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain M-M16T was 37.4 mol%. The phylogenetic and chemotaxonomic data and other phenotypic properties revealed that strain M-M16T represents a novel genus and species within the family Flavobacteriaceae, for which the name Sabulilitoribacter multivorans gen. nov., sp. nov. is proposed. The type strain of S. multivorans is M-M16T (= KCTC 32326T = CCUG 63831T).

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Acknowledgments

This work was supported by the Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR) and the Program for Collection, Management and Utilization of Biological Resources and BK21 program from the Ministry of Education, Science & Technology (MEST) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Yong-Taek Jung & Jung-Hoon Yoon

  2. Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Taejon, South Korea

    Jung-Sook Lee & Kenu-Chul Lee

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  1. Sooyeon Park
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  2. Yong-Taek Jung
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Correspondence to Jung-Hoon Yoon.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain M-M16T is KC618395.

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Park, S., Jung, YT., Lee, JS. et al. Sabulilitoribacter multivorans gen. nov., sp. nov., a polysaccharide-degrading bacterium of the family Flavobacteriaceae isolated from seashore sand. Antonie van Leeuwenhoek 104, 973–981 (2013). https://doi.org/10.1007/s10482-013-0016-0

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