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The use of parsimony network analysis for the formal delineation of phylogenetic species of yeasts: Candida apicola, Candida azyma, and Candida parazyma sp. nov., cosmopolitan yeasts associated with floricolous insects

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Abstract

Parsimony network analysis of rDNA sequences was used to delimit phylogenetic species of yeasts in an objective, formal manner. Many strains assigned to Candida apicola (Starmerella clade), when compared to the type, fell outside the inclusion limits proposed by Kurtzman and Robnett (1998) based on a pair-wise comparison of the large subunit rRNA gene D1/D2 domains. However, when these sequences were analyzed jointly with ITS rDNA sequences by parsimony network analysis, 28 of the 30 strains formed a cohesive set. Two strains, MUCL 45721 and CBS 4353, were excluded from the species, but there was no evident justification to subdivide the rest. A similar analysis of 81 isolates originally assigned to Candida azyma (Wickerhamiella clade) yielded dramatically different results, giving rise to six independent networks corresponding to Candida azyma sensu stricto (18 strains), Candida azymoides (2 strains), a pair of isolates from Australian hibiscus flowers, a single isolate from the same substrate, a single isolate from Malaysian bertam palm nectar, and 57 isolates that are assigned to the new species Candida parazyma (type = UWOPS 91-652.1T = CBS 11563T = NRRL Y-48669T). The strains retained in C. azyma sensu stricto differed from one another by up to four substitutions in their D1/D2 sequences, but their polymorphism at the level of the ITS was considerable and suggested a history of divergence resulting from dispersal. Strains of C. parazyma fell into seven variant haplotypes based on sequences of the rDNA ITS and D1/D2 regions. The most abundant haplotype occurred across the global range of the species. Others were either endemic to Belize, Costa Rica, Rarotonga, or Tennessee, suggestive of vicariance, or occurred across remote localities, offering partial support to the notion of rapid dispersal.

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Acknowledgments

This work was funded by the Natural Science and Engineering Research Council of Canada. We acknowledge the gift of strains from the Centraalbureau voor Schimmelcultures, The Netherlands (V. Robert, M. Groenewald), the Mycothèque de l’Université catholique de Louvain, Belgium (H.M. Daniel), the BIOTEC Culture collection, Thailand (S. Jindamorakot), as well as the research collections of C.A. Rosa and W.T. Starmer. We also benefited from the field collaboration of numerous individuals, including J.S.F. Barker, J.M. Bowles, D.H. Janzen, J. McNeil, M. Polak, and others. Special thanks are owed to C. Harding for the Belize collection.

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  1. Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada

    Marc-André Lachance, Jessica Dobson, Dilini N. Wijayanayaka & Allison M. E. Smith

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  1. Marc-André Lachance
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  2. Jessica Dobson
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  3. Dilini N. Wijayanayaka
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  4. Allison M. E. Smith
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Correspondence to Marc-André Lachance.

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Lachance, MA., Dobson, J., Wijayanayaka, D.N. et al. The use of parsimony network analysis for the formal delineation of phylogenetic species of yeasts: Candida apicola, Candida azyma, and Candida parazyma sp. nov., cosmopolitan yeasts associated with floricolous insects. Antonie van Leeuwenhoek 97, 155–170 (2010). https://doi.org/10.1007/s10482-009-9399-3

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