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A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei

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Abstract

In this study, a β-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358–601, respectively) were identified; the two domains are connected with a 84-amino-acids linker. The catalytic region showed an extensive sequence homology with other fungal β-glucosidases classified as family 3 glycoside hydrolases. The isolated Rhizomucor gene was expressed in the related fungus Mucor circinelloides. Transformant Mucor strains maintained the introduced plasmid in an autoreplicative manner and showed significantly higher cellobiase activity than the recipient strain.

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Acknowledgments

The research was supported by the ETT 214/2006 grant of the Hungarian Medical Research Council. We wish to thank the Proteomics Research Group at the Biological Research Center of the Hungarian Academy of Sciences for the LC–MS analysis of the BGL protein.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726, Szeged, Közép fasor 52, Hungary

    Miklós Takó, Adél Tóth, László G. Nagy, Csaba Vágvölgyi & Tamás Papp

  2. Department of Food Engineering, Faculty of Engineering, University of Szeged, Szeged, Hungary

    Judit Krisch

Authors
  1. Miklós Takó
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  2. Adél Tóth
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  3. László G. Nagy
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  4. Judit Krisch
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  5. Csaba Vágvölgyi
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  6. Tamás Papp
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Correspondence to Tamás Papp.

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Takó, M., Tóth, A., G. Nagy, L. et al. A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei . Antonie van Leeuwenhoek 97, 1–10 (2010). https://doi.org/10.1007/s10482-009-9382-z

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  • DOI: https://doi.org/10.1007/s10482-009-9382-z

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