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Nonomuraea aegyptia sp. nov., a novel actinomycete isolated from a sand dune

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Abstract

The taxonomic position of an unknown actinomycete isolated from a sand dune soil sample collected at Borg El-Arab in Egypt was established using a combination of genotypic and phenotypic data. Isolate S136T had chemotaxonomic and morphological properties consistent with its classification in the genus Nonomuraea and formed a distinct phyletic line in the Nonomuraea 16S rRNA gene tree. It was most closely related to the type strains of Nonomuraea helvata, Nonomuraea kuesteri and Nonomuraea turkmeniaca, sharing 16S rRNA gene similarities with these species of 97.1, 97.2 and 97.3%, respectively. The organism was distinguished from representatives of validly described Nonomuraea species using a range of phenotypic properties. It is apparent that the isolate belongs to a novel Nonomuraea species. The name proposed for this taxon is Nonomuraea aegyptia sp. nov., the type strain is S136T (=CGMCC 4.2054T = DSM 45082T).

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References

  • Chiba S, Suzuki M, Ando K (1999) Taxonomic re-evaluation of ‘Nocardiopsis’ sp. K-252T (= NRRL 15532T): a proposal to transfer this strain to the genus Nonomuraea as Nonomuraea longicatena sp. nov. Int J Syst Bacteriol 49:1623–1630

    PubMed  Google Scholar 

  • Chun J, Goodfellow M (1995) A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245

    PubMed  CAS  Google Scholar 

  • Collins CH, Lyne PM, Grange JM, Falkinham JO III (eds) (2004) Collins & Lyne’s microbiological methods, 8th edn. Arnold, London, pp 97–98

    Google Scholar 

  • Collins MD (1985) Isoprenoid quinone analysis in bacterial classification and identification. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 267–287

    Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Felsenstein J (1993) PHYLIP (phylogenetic inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA

    Google Scholar 

  • Fischer A, Kroppenstedt RM, Stackebrandt E (1983) Molecular genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis. J Gen Microbiol 129:3433–3446

    PubMed  CAS  Google Scholar 

  • Fitch WM, Margoliash E (1967) Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome C sequences is one of general applicability. Science 155:279–284

    Article  PubMed  CAS  Google Scholar 

  • Goodfellow M, Maldonado L, Quintana E (2005) Reclassification of Nonomuraea flexuosa (Meyer 1989) Zhang et al. (1998) as Thermopolyspora flexuosa gen. nov., comb. nov., nom. rev. Int J Syst Evol Microbiol 55:1979–1983

    Article  PubMed  CAS  Google Scholar 

  • Goodfellow M, Quintana E (2006) The family Streptosporangiaceae. In: Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E (eds) The Prokaryotes. Archaea. Bacteria: Firmicutes, Actinomycetes, vol 3. Springer, New York, pp 725–753

    Google Scholar 

  • Goodfellow M, Stanton LJ, Simpson KE, Minnikin DE (1990) Numerical and chemical classification of Actinoplanes and related actinomycetes. J Gen Microbiol 136:19–36

    Google Scholar 

  • Gordon RE, Mihm JM (1962) Identification of Nocardia caviae (Erikson) nov. com. Ann NY Acad Sci USA 98:628–636

    Article  Google Scholar 

  • Gyobu Y, Miyadoh S (2001) Proposal to transfer Actinomadura carminata to a new subspecies of the genus Nonomuraea as Nonomuraea roseoviolacea subsp. carminata comb nov. Int J Syst Evol Microbiol 51:881–889

    PubMed  CAS  Google Scholar 

  • Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322

    CAS  Google Scholar 

  • Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams & Wilkins, Baltimore

    Google Scholar 

  • Jones KL (1949) Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145

    PubMed  CAS  Google Scholar 

  • Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism, vol 3. Academic Press, New York, pp 21–123

    Google Scholar 

  • Kämpfer P, Kroppenstedt RM, Grün-Wollny I (2005) Nonomuraea kuesteri sp. nov. Int J Syst Evol Microbiol 55:847–851

    Article  PubMed  CAS  Google Scholar 

  • Kawato M, Shinobu R (1959) On Streptomyces herbaricolor sp. nov., supplement: a simple technique for microscopical observation. Mem Osaka Univ Lib Arts Educ B Nat Sci 8:114–119

    Google Scholar 

  • Kim SB, Brown R, Oldfield C, Gilbert SC, Iliarionov S, Goodfellow M (2000) Gordonia amicalis, sp. nov., a new dibenzothiophene desulphurizing actinomycete. Int J Syst Evol Microbiol 50:2031–2036

    PubMed  CAS  Google Scholar 

  • Kluge AG, Farris FS (1969) Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32

    Article  Google Scholar 

  • Kroppenstedt RM (1985) Fatty acid and menaquinone analysis of actinomycetes and related organisms. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 173–199

    Google Scholar 

  • Kroppenstedt RM, Goodfellow M (1991) The family Thermomonosporaceae. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes, 2nd edn. Springer, New York, pp 1085–1114

    Google Scholar 

  • Lechevalier HA, Lechevalier MP (1970) A critical evaluation of the genera of aerobic actinomycetes. In: Prauser H (ed) The Actinomycetales. VEB Gustav Fischer, Jena, pp 393–405

    Google Scholar 

  • Lechevalier MP, De Bièvre C, Lechevalier HA (1977) Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260

    Article  CAS  Google Scholar 

  • Meyer J (1989) Genus Actinomadura, Lechevalier and Lechevalier (1970a), 400AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2511–2526

    Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Mincer TJ, Gensen PR, Kauffman CA, Fenical W (2002) Widespread and persistent populations of a major new marine actinomycete taxon in ocean sediments. Appl Environ Microbiol 68:5005–5011

    Article  PubMed  CAS  Google Scholar 

  • O’Donnell AG, Falconer C, Goodfellow M, Ward AC, Williams E (1993) Biosystematics and diversity amongst novel carboxydotrophic actinomycetes. Antonie van Leeuwenhoek 64:325–340

    Article  PubMed  Google Scholar 

  • Poschner J, Kroppenstedt RM, Fischer A, Stackebrandt E (1985) DNA:DNA reassociation and chemotaxonomic studies on Actinomadura, Microbispora, Microtetraspora, Micropolyspora and Nocardiopsis. Syst Appl Microbiol 6:264–270

    CAS  Google Scholar 

  • Quintana E, Maldonado L, Goodfellow M (2003) Nonomuraea terrinata sp. nov., a novel soil actinomycete. Antonie van Leeuwenhoek 84:1–6

    Article  PubMed  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Stackebrandt E, Wink J, Steiner U, Kroppenstedt RM (2001) Nonomuraea dietzii sp. nov. Int J Syst Evol Microbiol 51:1437–1441

    PubMed  CAS  Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231

    PubMed  CAS  Google Scholar 

  • Sutcliffe IC (2000) Characterisation of a lipomannan lipoglycan from the mycolic acid containing actinomycete Dietzia maris. Antonie van Leeuwenhoek 78:195–201

    Article  PubMed  CAS  Google Scholar 

  • Tamura T, Suzuki S-I, Hatano K (2000) Acrocarpospora gen. nov., a new genus of the order Actinomycetales. Int J Syst Bacteriol 50:1163–1171

    CAS  Google Scholar 

  • Stackebrandt E, Rainey FA, Ward-Rainey NL (1997) Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacterial 47:479–491

    Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, 5 other authors (1987) Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Google Scholar 

  • Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA, Sackin MJ (1983) Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813

    PubMed  CAS  Google Scholar 

  • Zhang ZS, Wang Y, Ruan JS (1998) Reclassification of Thermomonospora and Microtetraspora. Int J Syst Bacteriol 48:411–422

    PubMed  Google Scholar 

  • Zhou ZH, Liu Z-H, Qian Y-D, Kim SB, Goodfellow M (1998) Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48:53–58

    PubMed  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Mrs Ros Brown for skilled technical assistance and to Dr Iain Sutcliffe (University of Northumbria) for help with the fatty acid analysis. MG is grateful for support from the UK Environmental Research Council (grant NER/T/S/2000/00616).

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Authors and Affiliations

  1. Botany Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt

    Wael N. Hozzein

  2. Division of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK

    Michael Goodfellow

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  1. Wael N. Hozzein
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  2. Michael Goodfellow
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Correspondence to Wael N. Hozzein.

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Hozzein, W.N., Goodfellow, M. Nonomuraea aegyptia sp. nov., a novel actinomycete isolated from a sand dune. Antonie van Leeuwenhoek 92, 165–171 (2007). https://doi.org/10.1007/s10482-007-9145-7

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