Abstract
The adverse toxicological profile of cisplatin (cis-dichlorodiammineplatinum (II)), characterized by nephrotoxicity and neurotoxicity is the main factor that limit the clinical usefulness of this antineoplastic drug, specifically the possibility of applying it in effective high-dose regimens. In order to overcome these disadvantages, many efforts in the search for new drugs have been made. Due to this particularity, we obtained via sol–gel process Pt(acac)2–TiO2 (NPt) nanostructured materials with antitumoral activity to be used as an alternative in the treatment of cancer tumors. The biocatalysts were prepared by the sol–gel route using the complex Pt(acac)2. Sol–gel parameters were controlled in order to obtain high platinum dispersion and particles in the nano-size range. TEM, FTIR, N2 adsorption and XPS characterization studies of the samples were carried out. In order to investigate interactions between the biocatalyst and DNA, agarose gel electrophoresis was performed, and we observed the formation of DNA adducts. 45 minutes after contact, NPt completely degraded the DNA (cisplatin 120 minutes). These results demonstrate that using a metal supported and dispersed over an inorganic biocompatible oxide, can be effectively used in the treatment of localized tumors.
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Abbreviations
- GBM:
-
Glioblastoma multiforme
- NPt:
-
platinum-titania nanoparticles
References
Beamson, G., Briggs, D.: High Resolution XPS of Organic Polymers. The Scienta ESCA 300 Database. Wiley, Chichester (1992)
Bosch, P., Lopez, T., Lara, V.-H., Gomez, R.: Pt/SiO2 catalysts: a comparison of the impregnation and the sol–gel methods. J. Mol. Catal. 80, 299–306 (1993)
Chen, G., Zhao, J., Liu, X., Gao, G., Huang, J., Li, G.: Electrochemical sensing DNA damage with nano-titanium dioxide and repair with a medicinal herb species resveratrol. J. Biotechnol. 127, 653–656 (2007)
Choi, H.-J., Kang, M.: Hydrogen production from methanol/water decomposition in a liquid photosystem using the anatase structure of Cu loaded TiO2. Int. J. Hydrog. Energy 32, 3841–3848 (2007)
Galanski, M., Baumgartner, C., Meelich, K., Arion, V.B., Fremuth, M., Jakupec, M.A., Schluga, P., Hartinger, C.G., Keyserlingk, N.G., Keppler, V.K.: Synthesis, crystal structure and pH dependent cytotoxicity of (SP-4-2)-bis(2-aminoethanolato-κ 2N,O)platinum(II)—a representative of novel pH sensitive anticancer platinum complexes. Inorg. Chim. Acta 357, 3237–3244 (2004)
Gao, J., Xua, B.: Applications of nanomaterials iside the cells. Nano Today 4, 37–51 (2009)
Gültekin, A., Diltemiz, S.E., Ersöz, A., Sarıözlü, N.Y., Denizli, A., Say, R.: Gold–silver nanoclusters having dipicolinic acid imprinted nanoshell for Bacillus cereus spores recognition. Talanta 78, 1332–1338 (2009)
Guzmán, A.M., Fernández, A.M., Franco, Y., Bautista, J.H., Rodríguez Páez, J.E.: Rev. Acad. Colomb. Cienc. 31(121), 529–536 (2007)
Hu, L., Boateng, K.A., Hill, J.M.: Sol–gel synthesis of Pt/Al2O3 catalysts: effect of Pt precursor and calcinations procedure on Pt dispersion. J. Mol. Catal. A, Chem. 259, 51–60 (2006)
Jadhav, V.B., Jun, Y.J., Song, J.H., Park, M.-K., Oh, J.H., Chae, S.W., Kim, I.-S., Choi, S.J., Lee, H.J., Sohn, Y.S.: A novel micelle-encasulated platinum(II) anticancer agent. J. Control. Release 147, 144–150 (2010)
Kaushik, A., Solanki, P.R., Ansari, A.A., Sumana, G., Ahmad, S., Malhotra, B.D.: Iron oxide-chitosan nanobiocomposite for urea sensor. Sens. Actuators B, Chem. 138, 572–580 (2009)
Klein, L.C. (ed.): Sol–gel technology for thin films, fiber performs, electronic and especiality shapes. Noyes, Park Ridge (1988)
Kuo, T.R., Wu, C.-L., Hsu, C.-H., Lo, W., Chiang, S.-J., Lin, S.-J., Dong, C.-Y., Chen, C.-C.: Chemical enhancer induced changes in the mechanisms of transdermal delivery of zinc oxide nanoparticles. Biomaterials 30, 3002–3008 (2009)
Lambert, C.K., Gonzalez, R.D.: The importance of measuring the metal content of supported metal catalysts prepared by the sol–gel method. Appl. Catal. A, Gen. 172, 233–239 (1998)
Lambert, S., Job, N., D’Souza, L., Ribeiro, Pereira M.F., Pirard, R., Heinrichs, B., Figuiredo, J.L., Pirard, J.-P., Regalbuto, J.R.: Synthesis of very highly dispersed platinum catalysts supported on carbon xerogelsby the strong electrostatic adsorption method. J. Catal. 261, 23–33 (2009)
Liu, L., Miao, P., Xu, Y., Tian, Z., Zou, Z., Li, G.: Study of Pt/TiO2 nanocomposite for cancer-cell treatment. J. Photochem. Photobiol., B Biol. 98, 207–210 (2010)
López, T., Recillas, S., Guevara, P., Sotelo, J., Alvarez, M., . Odriozola, J.A.: Pt/TiO2 brain biocompatible nanoparticles: GBM treatment using the C6 model in Wistar rats. Acta Biomater. 4, 2037–2044 (2008)
López, T., Figueras, F., Manjarrez, J., Bustos, J., Alvarez, M., Silvestre-Albero, J., Rodríguez-Reinoso, F., Martínez-Ferre, A., Martínez, E.: Catalytic nanomedicine: a new field in antitumor treatment using supported platinum nanoparticles. In vitro DNA degradation and in vivo tests with C6 animal model on Wistar rats. Eur. J. Med. Chem. 45, 1982–1990 (2010)
Pang, S.-K., Yu, C.-W., Au-Yeung, S., Ho, Y.-P.: DNA damage induced by novel demethylcantharidin-integrated platinum anticancer complexes. Biochem. Biophys. Res. Commun. 363, 235–240 (2007)
Park, J.H., Lee, S., Kim, J.-H., Park, K., Kim, K., Kwon, I.C.: Polymeric nanomedicine for cancer therapy. Prog. Polym. Sci. 33, 113–137 (2008)
Park, J.T., Seo, J.A., Ahn, S.H, Kim, J.H., Kang, S.W.: Surface modification of silica nanoparticles with hydrophilic polymers. J. Ind. Eng. Chem. 16, 517–522 (2010)
Patil, Y.B., Toti, U.S., Khdair, A., Ma, L., Panyam, J.: Single step surface functionalization of polymeric nanoparticles for targeted drug delivery. Biomaterials 30, 859–866 (2009)
Pirozzi, D., Fanelli, E., Aronne, A., Pernice, P., Mingione, A.: Lipase entrapment in a zirconia matrix: Sol–gel synthesis and catalytic properties. J. Mol. Catal., B Enzym. 59, 116–120 (2009)
Qiu, J., Wang, G.-J.: Comparative study on modification of multi-walled carbon nanotubes by a hydrophilic polymer with different approaches. Appl. Surf. Sci. 254, 5691–5694 (2008)
Sánchez, E., López, T., Gómez, R., Bokhimi, X., Morales, A., Novaro, O.: Synthesis and characterization of sol–gel Pt/TiO2 catalysts. J. Solid State Chem. 122, 309–314 (1996)
Schubert, U.: Chemical modification of titanium alkoxides for sol–gel processing. J. Mater. Chem. 15, 3701–3715 (2005)
Song, C., Ye, Z., Wang, G., Jin, D., Yuan, J., Guan, Y., Piper, J.: Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles. Talanta 79, 103–108 (2009)
Trewyn, B.G., Nieweg, J.A., Zhao, Y., Lin, V.S.-Y.: Biocompatible mesoporous silica nanoparticles with different morphologies for animal cell membrane penetration. Chem. Eng. J. 137, 23–29 (2008)
Zhou, L., Zhou, J.-H., Dong, C., Ma, F., Wei, S.-H., Shen, J.: Water-soluble hypocrellin A nanoparticles as a photodynamic therapy delivery system. Dyes Pigm. 82, 90–94 (2009)
Zhu, X., Chen, Z., Zhang, X., Zhu, Z., Li, G.: Biomolecule-directed assembly of binary gold and titanium dioxide nanoparticles. J. Nanosci. Nanotechnol. 10, 1021–1024 (2010)
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López, T., Alvarez, M., González, R.D. et al. Synthesis, characterization and in vitro cytotoxicity of Pt-TiO2 nanoparticles. Adsorption 17, 573–581 (2011). https://doi.org/10.1007/s10450-011-9330-x
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DOI: https://doi.org/10.1007/s10450-011-9330-x