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Nondestructive Techniques to Evaluate the Characteristics and Development of Engineered Cartilage

  • Nondestructive Characterization of Biomaterials for Tissue Engineering and Drug Delivery
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Abstract

In this review, methods for evaluating the properties of tissue engineered (TE) cartilage are described. Many of these have been developed for evaluating properties of native and osteoarthritic articular cartilage. However, with the increasing interest in engineering cartilage, specialized methods are needed for nondestructive evaluation of tissue while it is developing and after it is implanted. Such methods are needed, in part, due to the large inter- and intra-donor variability in the performance of the cellular component of the tissue, which remains a barrier to delivering reliable TE cartilage for implantation. Using conventional destructive tests, such variability makes it near-impossible to predict the timing and outcome of the tissue engineering process at the level of a specific piece of engineered tissue and also makes it difficult to assess the impact of changing tissue engineering regimens. While it is clear that the true test of engineered cartilage is its performance after it is implanted, correlation of pre and post implantation properties determined non-destructively in vitro and/or in vivo with performance should lead to predictive methods to improve quality-control and to minimize the chances of implanting inferior tissue.

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Acknowledgments

Research reported in this publication was supported by the National Institute of Biomedical Imaging and Bioengineering under Award Number R01 EB20367-01. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors have no financial relationships that may cause a conflict of interest.

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Authors and Affiliations

  1. Departments of Mechanical and Aerospace Engineering, Case Western Reserve University, 2123 Martin Luther King Jr. Drive, Glennan Building Room 616A, Cleveland, OH, 44106, USA

    Joseph M. Mansour

  2. Radiology and Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA

    Zhenghong Lee

  3. Biology (Skeletal Research Center), Case Western Reserve University, Cleveland, OH, USA

    Jean F. Welter

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  1. Joseph M. Mansour
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  2. Zhenghong Lee
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  3. Jean F. Welter
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Correspondence to Joseph M. Mansour.

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Associate Editor Rebecca Kuntz-Willits oversaw the review of this article.

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Mansour, J.M., Lee, Z. & Welter, J.F. Nondestructive Techniques to Evaluate the Characteristics and Development of Engineered Cartilage. Ann Biomed Eng 44, 733–749 (2016). https://doi.org/10.1007/s10439-015-1535-9

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