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Phospholipid Polymer Biointerfaces for Lab-on-a-Chip Devices

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Abstract

This review summarizes recent achievements and progress in the development of various functional 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer biointerfaces for lab-on-a-chip devices and applications. As phospholipid polymers, MPC polymers can form cell-membrane-like surfaces by surface chemistry and physics and thereby provide biointerfaces capable of suppressing protein adsorption and many subsequent biological responses. In order to enable application to microfluidic devices, a number of MPC polymers with diverse functions have been specially designed and synthesized by incorporating functional units such as charge and active ester for generating the microfluidic flow and conjugating biomolecules, respectively. Furthermore, these polymers were incorporated with silane or hydrophobic moiety to construct stable interfaces on various substrate materials such as glass, quartz, poly(methyl methacrylate), and poly(dimethylsiloxane), via a silane-coupling reaction or hydrophobic interactions. The basic interfacial properties of these interfaces have been characterized from multiple aspects of chemistry, physics, and biology, and the suppression of nonspecific bioadsorption and control of microfluidic flow have been successfully achieved using these biointerfaces on a chip. Further, many chip-based biomedical applications such as immunoassays and DNA separation have been accomplished by integrating these biointerfaces on a chip. Therefore, functional phospholipid polymer interfaces are promising and useful for application to lab-on-a-chip devices in biomedicine.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas ‘‘Molecular Soft-Interface Science’’ from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The authors thank Prof. Michael Detamore, The University of Kansas, for providing the opportunity to contribute this review paper in the special issue of this journal.

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  1. Yan Xu

    Present address: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan

Authors and Affiliations

  1. Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan

    Yan Xu, Madoka Takai & Kazuhiko Ishihara

  2. Center for NanoBio Integration, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan

    Yan Xu, Madoka Takai & Kazuhiko Ishihara

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  1. Yan Xu
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  2. Madoka Takai
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  3. Kazuhiko Ishihara
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Correspondence to Yan Xu or Madoka Takai.

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Associate Editor Michael S. Detamore oversaw the review of this article.

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Xu, Y., Takai, M. & Ishihara, K. Phospholipid Polymer Biointerfaces for Lab-on-a-Chip Devices. Ann Biomed Eng 38, 1938–1953 (2010). https://doi.org/10.1007/s10439-010-0025-3

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