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Study on Tumor Microvasculature Damage Induced by Alternate Cooling and Heating

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Abstract

Tumor vasculature damage induced by various thermal treatments has been studied in vivo via laser confocal microscopy. Murine mammary carcinoma 4T1 was implanted in the nude mice dorsal skin fold window chamber. The implanted tumor was treated by alternate cooling and heating. Results showed that the treatment was much more effective as compared with that of cooling or heating alone, especially in damaging the tumor vasculature. In general, tumor vascular response to thermal stimuli was heterogeneous. All the treatments of hyperthermia at 42 °C (for 1 h), alternate cooling at 1 °C and heating at 42 °C (for 1/2 h each) and that of −10 °C/42 °C (for 1/2 h each) enhanced liposome extravasation. Pre-cooling tumor at 1 °C preserved most of the vascular integrity but partially inhibited the effect of post-hyperthermia at 42 °C. On the other hand, cooling at −10 °C for 1/2 h before heating at 42 °C caused severe vessel damage. Histo-pathological analyses further confirmed the effect as rare tumor vessel recurrence and large necrotic tumor tissue areas shown on the 7th day after the treatment.

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Acknowledgment

This work has been supported by the National Natural Science Foundation of China (50436030, 50506016, 50725622).

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong Chuan Rd., Shanghai, 200240, People’s Republic of China

    Yuanyuan Shen, Ping Liu, Aili Zhang & Lisa X. Xu

  2. Med-X Research Institute, Shanghai Jiao Tong University, 800 Dong Chuan Rd., Shanghai, 200240, People’s Republic of China

    Lisa X. Xu

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  1. Yuanyuan Shen
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Correspondence to Lisa X. Xu.

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Shen, Y., Liu, P., Zhang, A. et al. Study on Tumor Microvasculature Damage Induced by Alternate Cooling and Heating. Ann Biomed Eng 36, 1409–1419 (2008). https://doi.org/10.1007/s10439-008-9511-2

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  • DOI: https://doi.org/10.1007/s10439-008-9511-2

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