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Evaluation of anti-adenoviral effects of the polyvinyl alcohol iodine ophthalmic solution

  • Laboratory Investigation
  • Published:
Japanese Journal of Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

To investigate the virucidal effects of a polyvinyl alcohol iodine, Saniode, against 16 types of human mastadenovirus (HAdV) causing ophthalmic, respiratory, gastrointestinal, urinary, and systemic infections.

Study design

Laboratory investigation

Methods

Fifty microliters of Saniode were exposed to 10 μL each containing HAdV virus stock solution of 1 × 106 copies/μL of HAdV-1, -2, -3, -4, 5, -6, -7, -8, -11, -37, -53, -54, -56, -64, -81, and -85 for 10 s, 30 s, 1 min, and 3 min. After neutralization with 0.5% sodium thiosulfate, the mixture was diluted by ten-fold serial dilution and inoculated into 24 wells containing confluent A549 cell monolayers. Virucidal effects were calculated relative to the positive control on days 7–10 and observed until 30 days post-infection.

Results

Saniode satisfied the EN-14476 criterion for virucidal effects (>99.99%) for all HAdV types at all exposure times, including at 10 s on days 7 to 10 post-infection. All types of HAdVs that reacted for > 1 min achieved 99.99% reduction, including after 30 days.

Conclusion

Saniode displayed virucidal effects against all tested HAdV types. Currently, with no specific medication available for HAdVs in ocular infection, this could be an option to prevent the spread of keratoconjunctivitis.

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Acknowledgements

The authors thank Editage (www. editage.jp) for the English language reviews. This study was supported by funding from Fukuoka University (Grant No. GW2212).

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Authors and Affiliations

  1. Department of Ophthalmology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan

    Tomoko Tsukahara-Kawamura & Eiichi Uchio

  2. Center for Emergency Preparedness and Response, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan

    Nozomu Hanaoka

Authors
  1. Tomoko Tsukahara-Kawamura
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  3. Eiichi Uchio
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Correspondence to Eiichi Uchio.

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T. Tsukahara-Kawamura, None; N. Hanaoka, None; E. Uchio, None.

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Corresponding Author: Eiichi Uchio

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Tsukahara-Kawamura, T., Hanaoka, N. & Uchio, E. Evaluation of anti-adenoviral effects of the polyvinyl alcohol iodine ophthalmic solution. Jpn J Ophthalmol 68, 64–69 (2024). https://doi.org/10.1007/s10384-023-01027-1

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  • DOI: https://doi.org/10.1007/s10384-023-01027-1

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