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Magnetic Solid-Phase Extraction Pretreatment Method for Quantitative Analysis of 12 Steroids in Human Serum Based on LC–MS/MS

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Abstract

Accurate quantification of steroid hormones based on LC–MS/MS is of great significance for the diagnosis of endocrine diseases and the study of metabolomics. However, the current liquid–liquid extraction (LLE) and solid-phase extraction (SPE) are difficult to be further accepted in the pretreatment of clinical samples. Therefore, we carefully developed and optimized a novel method for the treatment of human serum samples by magnetic solid-phase extraction (MSPE). Several necessary tests for 12 steroids, such as LLOQ, matrix effect, recovery and precision, have verified the repeatability and practicability of the method. The comparison with LLE proves that the environment-friendly MSPE has the significance of further popularization in the treatment of clinical samples. Focusing on the future, the study opens the door for the automatic preprocessing of human serum samples based on MSPE, including but not limited to the quantitative analysis of steroid hormones.

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Data Availability Statement

All data included in this study are available upon request by contact with the corresponding author.

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Acknowledgements

This work was supported by National Key Research and Development Program of China under grand No. 2021YFC2401100, Key Project of Science and Technology to Boost the Economy in 2020 under grand No. SQ2020YFF0423480, Science and Technology Service Network Initiative of Chinese Academy of Sciences under grand No. KFJ-STS-QYZD-188, Scientific Instrument Developing Project of Chinese Academy of Sciences under grand No. ZDKYYQ20180003. Science and Technology Planning Project of Tianjin under grand No. 22YFYSHZ00140.

Funding

National Key Research and Development Program of China, 2021YFC2401100, Key Project of Science and Technology to Boost the Economy in 2020, SQ2020YFF0423480, Science and Technology Service Network Initiative of Chinese Academy of Sciences, KFJ-STS-QYZD-188, Scientific Instrument Developing Project of Chinese Academy of Sciences, ZDKYYQ20180003, Science and Technology Planning Project of Tianjin, 22YFYSHZ00140.

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Author notes

  1. Yanjie Li, Tianyi Wang have contributed equally to this work.

Authors and Affiliations

  1. Tianjin Guoke Medical Engineering and Technology Development Co., Ltd, Tianjin, 300300, China

    Yanjie Li, Tianyi Wang, Suzi Qin, Yan Li, Wei Hu & Wenbo Cheng

  2. Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China

    Yan Li, Wei Hu & Wenbo Cheng

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  1. Yanjie Li
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  2. Tianyi Wang
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  3. Suzi Qin
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  4. Yan Li
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  5. Wei Hu
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  6. Wenbo Cheng
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Contributions

All authors contributed to the study conception and design, material preparation, data collection and analysis. The first draft of the manuscript was written by YL and all authors commented on previous versions of the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Wenbo Cheng.

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10337_2023_4244_MOESM1_ESM.docx

Supporting information: The concentrations of calibrators and QC samples, as well as the detailed data of method optimization and validation, can be found in the supporting information. Supplementary file1 (DOCX 177 KB)

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Li, Y., Wang, T., Qin, S. et al. Magnetic Solid-Phase Extraction Pretreatment Method for Quantitative Analysis of 12 Steroids in Human Serum Based on LC–MS/MS. Chromatographia 86, 333–347 (2023). https://doi.org/10.1007/s10337-023-04244-5

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  • DOI: https://doi.org/10.1007/s10337-023-04244-5

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