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The Pharmacokinetics and Bio-distribution Studied by Simultaneous Analysis of Curcumin and Paclitaxel in Biosamples

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Abstract

A simple, rapid, sensitive, robust and reproducible and specific high-performance liquid chromatography (HPLC) analysis method for the simultaneous determination of Curcumin (CU) and Paclitaxel (PTX) was developed and validated. After pretreating with citric acid/trisodium citrate (CA) buffer (pH 3.6) as stabilizer and extracting from the biosamples with ethyl acetate/methanol (90/10, v/v), CU and PTX were chromatographed on a Luna C18 column (4.6 mm × 250 mm, 5 µm) at a flow rate of 1 mL·min−1 with acetonitrile (A)/triethylamine phosphate buffer (pH 3.5; 0.012 M) (B) as the mobile phase by variable wavelength and gradient elution method of 0–9.5 (min)/55% A–45% B at 425 nm, 9.51–11.5 (min)/40% A–60% B at 227 nm, 11.51–19.0 (min)/55% A–45% B at 425 nm. The method was validated related to linearity, accuracy, precision and stability. After pretreatment, CU and PTX were stable in biological samples at room temperature for 24 h and at – 20 °C storage for 7 days. Besides, compared to CU or PTX alone, an altered pharmacokinetics in rabbits and bio-distribution behavior in rats by co-administration were observed, and its influence rules and mechanisms still need further study.

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Acknowledgements

This study was supported by the Youth Science and technology innovation research team(No.2021JDTD0008) and basic research fund (No.2020YJ0336, 2020YJ0373) of the science and technology department of Sichuan province of China, the Joint Fund of Luzhou City and Southwest Medical University [No.2019LZXNYDZ07], the Science and Technology Fund of Luzhou science and technology and Human Resources Bureau (No. 2019-SYF-35), Science and Technology Innovation Team from Jiucheng Science and Technology Talent Cultivation Plan in Luzhou City (No. 2019-1).

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  1. Chao Pi and Lanmei Li have contributed equally to this work.

Authors and Affiliations

  1. Central Nervous System Drug Key Laboratory of Sichuan Province, Southwest Medical University, Luzhou, 646000, Sichuan, PR China

    Chao Pi, Lanmei Li & Shaoqiu Huang

  2. Nanchong Key Laboratory of Individualized Drug Therapy, Department of Pharmacy, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, Nanchong, Sichuan, PR China

    Lanmei Li

  3. The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, PR China

    Shaozhi Fu & Hongru Yang

  4. Central Nervous System Drug Key Laboratory of Sichuan Province, The Affiliated Hospital of Traditional Chinese Medicine of Southwest Medical University, Luzhou, 646000, Sichuan, PR China

    Yonggen Zou, Ling Zhao & Yumeng Wei

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  1. Chao Pi
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Pi, C., Li, L., Fu, S. et al. The Pharmacokinetics and Bio-distribution Studied by Simultaneous Analysis of Curcumin and Paclitaxel in Biosamples. Chromatographia 84, 889–904 (2021). https://doi.org/10.1007/s10337-021-04076-1

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  • DOI: https://doi.org/10.1007/s10337-021-04076-1

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