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De Novo Design of a Cyclic Polyhistidine Peptide for Binding with Quantum Dots: Self-Assembly Investigation Using Capillary Electrophoresis

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Abstract

In this study, a cyclic disulfide-bonded peptide (ATTO 590-cyclo(2,11)-HCHVH DPLPHLHCH, clyco(ATTO-HCHV) was designed and synthesized. The β-turn design by the dipeptide d-Pro-l-Pro and the intradisulfide bridge using two cysteines make all histidines residues align on one face of the peptide, suggesting less steric hindrance during the self-assembly with quantum dots (QDs) and higher binding affinity than histag. QDs and clyco(ATTO-HCHV) at different molar ratio were sequentially injected into the capillary; strong Förster resonance energy transfer (FRET) signals were observed in both donor and acceptor channels, indicating the efficient binding of the novel cyclic peptide ligand onto the QDs to form clyco(ATTO-HCHV)-QD assembly inside the capillary. Capillary electrophoresis coupled with fluorescence detection (CE-FL) results indicated that the cyclic ligand clyco(ATTO-HCHV) had a much higher binding affinity than that of the linear form peptide ligand ATTO-HCHV. Additionally, the interval time of the injection and the sampling time were also investigated using CE-FL combining with FRET technology. Lastly, the stability of clyco(ATTO-HCHV)-QD was systematically examined in the presence of the imidazole competitor. It is believed that this new in-capillary assay significantly reduced the sample consumption and the analysis time.

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Acknowledgements

This work was supported by the National Natural Science Foundation (Grant Number 21602020), the Natural Science Foundation of Jiangsu Province (Grant No. BK20141170), the Project of Jiangsu Province Industry-University-Research joint innovation fund (Grant No. BY2016029-22) and the International Scientific Cooperation Project of Changzhou Scientific Bureau (Grant No. CZ20160015). This work was also supported by the Advanced Catalysis and Green Manufacturing Collaborative Innovation Center of Changzhou University, and the 333 Project of Jiangsu Province.

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Authors and Affiliations

  1. School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, 213164, Jiangsu, China

    Lin Qiu, Chencheng Zhang, Ting Gu, Zhilan Zhu, Jianpeng Wang, Li Liu, Shumin Ding, Xiaoqian Liu, Jianhao Wang & Pengju Jiang

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  1. Lin Qiu
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  2. Chencheng Zhang
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  3. Ting Gu
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  4. Zhilan Zhu
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  5. Jianpeng Wang
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  6. Li Liu
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  8. Xiaoqian Liu
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  9. Jianhao Wang
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Correspondence to Jianhao Wang or Pengju Jiang.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Published in the topical collection Peptide and Protein Analysis with Debby Mangelings and Gerhard K. E. Scriba as editors.

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Qiu, L., Zhang, C., Gu, T. et al. De Novo Design of a Cyclic Polyhistidine Peptide for Binding with Quantum Dots: Self-Assembly Investigation Using Capillary Electrophoresis. Chromatographia 81, 41–46 (2018). https://doi.org/10.1007/s10337-017-3319-x

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  • DOI: https://doi.org/10.1007/s10337-017-3319-x

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