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On-Line HPLC with Biochemical Detection for Screening Bioactive Compounds in Complex Matrixes

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An Erratum to this article was published on 11 February 2016

Abstract

On-line high-performance liquid chromatography (HPLC) coupled with biochemical detection (BCD) has been developed to screen compounds showing antioxidant action, enzyme inhibition and receptor affinity in complex matrixes. This review summarizes HPLC methods combining different post-column detection methods, such as diode-array detection (DAD), mass spectrometry (MS), chemiluminescence (CL) and nuclear magnetic resonance, for antioxidant screening. The methods based on a single relatively stable reagent such as DPPH• and ABTS•+ were the most popular. Oxygen free radical scavengers mainly depended on post-column CL detection. The on-line hyphenated HPLC–BCD systems based on post-column UV/DAD fluorescence and MS detection were also widely applied to screen enzyme- and receptor-active compounds. These strategies provide a convenient tool for quick identification and quantification of active compounds in complex matrixes.

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Acknowledgments

This work was supported by the National Scientific Foundation of China (No. 81403903) and Hubei Provincial Scientific Foundation (No. 2015CFB493).

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  1. Hubei Institution of Traditional Chinese Medicine Control, Hubei Institution for Food and Drug Control, Dingziqiaolu 54#, Wuhan, 430064, China

    Wen-Bin Peng, Jing-Ling Tan, Dan-Dan Huang & Xiao-Ping Ding

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  1. Wen-Bin Peng
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Correspondence to Xiao-Ping Ding.

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Peng, WB., Tan, JL., Huang, DD. et al. On-Line HPLC with Biochemical Detection for Screening Bioactive Compounds in Complex Matrixes. Chromatographia 78, 1443–1457 (2015). https://doi.org/10.1007/s10337-015-2982-z

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