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Imaging E-selectin expression following traumatic brain injury in the rat using a targeted USPIO contrast agent

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Magnetic Resonance Materials in Physics, Biology and Medicine Aims and scope Submit manuscript

Abstract

Introduction

The aim of this work was to map E-selectin expression in a traumatic brain injury model using a newly-designed MR contrast agent. Iron cores, responsible for susceptibility effects and therefore used as T2* contrast agents, need to be coated in order to be stabilized and need to be targeted to be useful.

Methods

We have designed a molecule coating composed, at one end, of bisphosphonate to ensure anchorage of the coating on the iron core and, at the other end, of Fukuda’s defined heptapeptide known to target selectin binding sites.

Conclusion

The synthesized nanoparticles were able to non-invasively target the traumatic brain lesion, inducing a specific T2* decrease of about 25% up to at least 70 min post-injection of the targeted contrast agent.

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Abbreviations

IL1-β and TNF-α :

Interleukin-1-β and tumor necrosis factor-α

IELLQAR:

Isoleucine-glutamate-leucine-leucine-glutamine-alanine-arginine heptapeptide

BP:

Bisphosphonate

99mTc-BP:

Technetium chelated to bisphosphonate

EG3:

Ethylene glycol block repeated three times

Hd:

Hydrodynamic diameter

ζ :

Zeta potential

e:

Polyelectrolyte accessible layer depth

d q :

Charge density (in C/m3)

RARE:

Rapid acquisition with relaxation enhancement

ADC:

Apparent diffusion coefficient

3DmGE:

3D multi-gradient echos sequence

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Authors and Affiliations

  1. INSERM U 646, Ingénierie de la Vectorisation Particulaire, 10, rue André Boquel, 49100, Angers, France

    Catherine Chapon, Franck Lacoeuille, François Hindré, Patrick Saulnier, Jean-Pierre Benoit, Jean-Jacques Le Jeune & Laurent Lemaire

  2. Université d’Angers, UMR-S646, 49100, Angers, France

    Catherine Chapon, Franck Lacoeuille, François Hindré, Patrick Saulnier, Jean-Pierre Benoit, Jean-Jacques Le Jeune & Laurent Lemaire

  3. Service Commun d’Analyses Spectroscopiques, Université d’Angers, Angers, France

    Florence Franconi

Authors
  1. Catherine Chapon
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  2. Florence Franconi
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  3. Franck Lacoeuille
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  8. Laurent Lemaire
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Correspondence to Laurent Lemaire.

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Chapon, C., Franconi, F., Lacoeuille, F. et al. Imaging E-selectin expression following traumatic brain injury in the rat using a targeted USPIO contrast agent. Magn Reson Mater Phy 22, 167–174 (2009). https://doi.org/10.1007/s10334-008-0161-z

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  • DOI: https://doi.org/10.1007/s10334-008-0161-z

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