Abstract
Objective
This study was to investigate whether PTEN mutations play a role in the carcinogenesis of soft tissue sarcomas (STS).
Methods
Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to amplify 4 exons of PTEN and to analyze the conformation polymorphism, then DNA sequencing methods was used to detect point mutation of PTEN gene four exons of abnormal single strand conformation in soft tissue sarcomas.
Results
Two of 86 cases showed 130th condon G→A missense mutation in the exon 8 of PTEN gene, and this mutation made Arg to change to Gln in PTEN protein structure 334th condon A→T missense mutation in the exon 8 of PTEN gene, and this mutation made Asn to change to Lys in PTEN protein structure.
Conclusion
These data indicated the existence of PTEN mutation in soft tissue sarcomas, but PTEN gene mutation rate is very low. PTEN mutation may plays an less important role in the development and malignant transformation of soft tissue sarcomas.
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Supported by a grant from the National Natural Science Foundation of China (No. 30560169).
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Yin, L., chen, Y., Li, W. et al. Mutational analysis of the PTEN gene in soft tissue sarcomas. Chin. -Ger. J. Clin. Oncol. 9, 422–425 (2010). https://doi.org/10.1007/s10330-010-0647-9
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DOI: https://doi.org/10.1007/s10330-010-0647-9