Abstract
Tomato leaf curl disease (ToLCD) affected 25% of the tomato crop in Chitrakoot, India and symptomatic leaves were collected for molecular assay. The complete sequences of bipartite begomovirus DNA-A and a betasatellite DNA were amplified. In a sequence analysis, begomovirus DNA-A and betasatellite shared highest sequence identity (91–99%) with Tomato leaf curl New Delhi virus (ToLCNDV) DNA-A and chili leaf curl betasatellite (ChLCB), respectively. The virus was transmitted by whitefly to tomato plants and caused ToLCD symptoms with 70% transmission rate. To our knowledge, this is the first report of the natural occurrence of ToLCNDV and ChLCB in India.
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Acknowledgements
The authors thank the university research laboratory and Prof. N. C. Gautam, Vice-Chancellor, Mahatma Gandhi Chitrakoot Gramoday Vishwavidyalaya (MGCGV), Chitrakoot, Satna, Madhya Pradesh, India for providing instrumentation, basic facilities and constant encouragement.
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Supplementary Materials Table S1: Primer details used in this study to detect the associated virus and satellites molecule (DOCX 76 KB)
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Agnihotri, A.K., Mishra, S.P., Tripathi, R.C. et al. First natural co-occurrence of tomato leaf curl New Delhi virus DNA-A and chili leaf curl betasatellite on tomato plants (Solanum lycopersicum L.) in India. J Gen Plant Pathol 84, 414–417 (2018). https://doi.org/10.1007/s10327-018-0807-2
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DOI: https://doi.org/10.1007/s10327-018-0807-2