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Practical method combining loop-mediated isothermal amplification and bait trap to detect Pythium helicoides from hydroponic culture solutions

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Abstract

Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the bait was used in the LAMP reaction, whereas in the “Bait culture-LAMP”, a crude extract of mycelia grown out from perilla seeds onto Pythium-selective medium served as the bait. The two methods are simple and rapid for practical monitoring of P. helicoides in hydroponic culture systems.

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Acknowledgements

This work was supported in part by Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industries from the Ministry of Agriculture, Forestry and Fisheries, Japan.

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Authors and Affiliations

  1. Aichi Agricultural Research Center, 1-1 Sagamine, Yazako, Nagakute, Aichi, 480-1193, Japan

    Noriyuki Miyake, Hirofumi Nagai, Shinro Kato, Masashi Matsusaki, Shiro Fukuta, Reiko Takahashi & Ryoji Suzuki

  2. River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Yasushi Ishiguro

Authors
  1. Noriyuki Miyake
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  2. Hirofumi Nagai
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  3. Shinro Kato
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  4. Masashi Matsusaki
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  5. Shiro Fukuta
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  6. Reiko Takahashi
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  7. Ryoji Suzuki
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  8. Yasushi Ishiguro
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Corresponding author

Correspondence to Noriyuki Miyake.

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Miyake, N., Nagai, H., Kato, S. et al. Practical method combining loop-mediated isothermal amplification and bait trap to detect Pythium helicoides from hydroponic culture solutions. J Gen Plant Pathol 83, 1–6 (2017). https://doi.org/10.1007/s10327-016-0691-6

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  • DOI: https://doi.org/10.1007/s10327-016-0691-6

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