Skip to main content
SpringerLink
Log in

A simple, efficient agroinoculation soaking procedure for Tomato yellow leaf curl virus

  • Technique
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Tomato yellow leaf curl virus (TYLCV, genus Begomovirus, family Geminiviridae), poses a serious threat to tomato crops in tropical and subtropical regions. We developed a simple agroinoculation method with an infectious clone of TYLCV. Dipping of excised sections of susceptible tomato shoots in an Agrobacterium suspension successfully introduced the replicating virus with high efficiency. An additional vacuum treatment for 5 min ensured uniform infection without escapes, allowing evaluation of differences in TYLCV resistance among tomato cultivars. The method can be used in laboratory experiments for virological studies and in breeding programs for resistant cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Al Abdallat AM, Al Debei HS, Asmar H, Misbeh S, Quraan A, Kvarnheden A (2010) An efficient in vitro-inoculation method for Tomato yellow leaf curl virus. Virol J 7:84

    Article  PubMed  Google Scholar 

  • Czosnek H, Kheyr-Pour A, Gronenborn B, Remetz E, Zeidan M, Altman A, Rabinowitch HD, Vidavsky S, Kedar N, Gafni Y, Zamir D (1993) Replication of Tomato yellow leaf curl virus (TYLCV) DNA in agroinoculated leaf discs from selected tomato genotypes. Plant Mol Biol 22:995–1005

    Article  PubMed  CAS  Google Scholar 

  • Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349

    Article  PubMed  CAS  Google Scholar 

  • Lapidot M (2007) Screening for TYLCV-resistant plants using whitefly-mediated inoculation. In: Czosnek H (ed) Tomato yellow leaf curl disease. Management, molecular biology, breeding for resistance. Springer, Dordrecht, pp 329–342

    Chapter  Google Scholar 

  • Mansoor S, Briddon RW, Zafar Y, Stanley J (2003) Geminivirus disease complexes: an emerging threat. Trends Plant Sci 8:128–134

    Article  PubMed  CAS  Google Scholar 

  • Moriones E, Navas-Castillo J (2000) Tomato yellow leaf curl virus, an emerging virus complex causing epidemics worldwide. Virus Res 71:123–134

    Article  PubMed  CAS  Google Scholar 

  • Ohnishi J, Kitamura T, Terami F, Honda K (2009) A selective barrier in the midgut epithelial cell membrane of the nonvector whitefly Trialeurodes vaporariorum to Tomato yellow leaf curl virus uptake. J Gen Plant Pathol 75:131–139

    Article  Google Scholar 

  • Picó B, Ferriol M, Díez MJ, Viñals FN (2001) Agroinoculation methods to screen wild Lycopersicon for resistance to Tomato yellow leaf curl virus. J Plant Pathol 83:215–220

    Google Scholar 

  • Ueda S, Kimura T, Onuki M, Hanada K, Iwanami T (2004) Three distinct groups of isolates of Tomato yellow leaf curl virus in Japan and construction of an infectious clone. J Gen Plant Pathol 70:232–238

    Article  Google Scholar 

  • Vidavski F, Czosnek H, Gazit S, Levy D, Lapidot M (2008) Pyramiding of genes conferring resistance to Tomato yellow leaf curl virus from different wild tomato species. Plant Breed 127:625–631

    Article  Google Scholar 

Download references

Acknowledgments

We thank Dr. Shigenori Ueda (NARO, KONARC, Japan) for providing the infectious clone pBSzY and useful suggestions on our study.

Author information

Authors and Affiliations

  1. NARO Institute of Vegetable and Tea Science, 360 Kusawa, Ano-cho, Tsu, Mie, 514-2392, Japan

    Hirotaka Yamaguchi, Jun Ohnishi, Koji Miyatake, Tsukasa Nunome, Akio Ohyama, Satomi Negoro & Hiroyuki Fukuoka

Authors
  1. Hirotaka Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jun Ohnishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Koji Miyatake
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tsukasa Nunome
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Akio Ohyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Satomi Negoro
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hiroyuki Fukuoka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hirotaka Yamaguchi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamaguchi, H., Ohnishi, J., Miyatake, K. et al. A simple, efficient agroinoculation soaking procedure for Tomato yellow leaf curl virus . J Gen Plant Pathol 79, 243–248 (2013). https://doi.org/10.1007/s10327-013-0450-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-013-0450-x

Keywords

Search

Navigation