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Analysis of genetic and biological characters of Japanese potato strains of Ralstonia solanacearum

  • Bacterial and Phytoplasma Diseases
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Abstract

We assessed the geographic distribution, biovar, phylotype, DNA fingerprints (rep-PCR), and/or endoglucanase sequence of potato bacterial wilt pathogen, Ralstonia solanacearum (Rs), in Japan. Rs has been isolated from potato fields in southwestern, warm, temperate regions. Of the 188 isolates, 74 belonged to biovar N2 (39%), 44 to biovar 3 (24%), and 70 to biovar 4 (37%). Biovars N2 and 4 strains were widely distributed, from northern (Hokkaido) to southern (Okinawa) Japan. Based on the results of multiplex-PCR analysis, every potato strains belonged to either phylotype I or IV. Phylotype I comprised both biovars 3 and 4 strains. On the other hand, phylotype IV included biovar N2 strains. None of the strains belonged to phylotype II or III or biovar 1 or 2. Phylogenetic analysis based on DNA fingerprints and endoglucanase gene sequences clarified the genetic diversity of the Japanese potato strains and the close genetic relationship between the Japanese strains and the Asian strains in phylotypes I and IV.

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Acknowledgments

Authors thank all of those who contributed the strains or supported our field survey in this study.

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Authors and Affiliations

  1. National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, 305-8604, Japan

    Mitsuo Horita

  2. Nagasaki Agricultural and Forestry Technical Development Center, Omura, Nagasaki, 856-0021, Japan

    Yasuhiro Suga

  3. Okinawa Prefectural Agricultural Research Center, Naha, Okinawa, 905-0012, Japan

    Atsushi Ooshiro

  4. Graduate School of Agriculture, Kyushu University, Fukuoka, Fukuoka, 812-8581, Japan

    Kenichi Tsuchiya

Authors
  1. Mitsuo Horita
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  2. Yasuhiro Suga
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  3. Atsushi Ooshiro
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  4. Kenichi Tsuchiya
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Corresponding author

Correspondence to Mitsuo Horita.

Additional information

Sequences of partial endoglucanase gene fragments have been deposited in DDBJ/EMBL/Genbank databases under the accession numbers AB523862-AB523878.

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Horita, M., Suga, Y., Ooshiro, A. et al. Analysis of genetic and biological characters of Japanese potato strains of Ralstonia solanacearum . J Gen Plant Pathol 76, 196–207 (2010). https://doi.org/10.1007/s10327-010-0229-2

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  • DOI: https://doi.org/10.1007/s10327-010-0229-2

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