Abstract
We transformed Colletotrichum trifolii, the causal agent of alfalfa anthracnose, using Agrobacterium tumefaciens as a new tool for random insertional mutagenesis. Fungal spores of C. trifolii were transformed with T-DNA including the hygromycin phosphotransferase gene (hph). Southern analysis showed that every randomly selected transformant had a unique hybridization pattern of T-DNA, suggesting that the T-DNA was randomly integrated into the fungal genome. More significantly, about 75% of transformants had a single copy of the T-DNA. The results demonstrate that insertional mutagenesis via A. tumefaciens is a useful tool for studying the function of C. trifolii genes.
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Takahara, H., Tsuji, G., Kubo, Y. et al. Agrobacterium tumefaciens-mediated transformation as a tool for random mutagenesis of Colletotrichum trifolii . J Gen Plant Pathol 70, 93–96 (2004). https://doi.org/10.1007/s10327-003-0099-y
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DOI: https://doi.org/10.1007/s10327-003-0099-y