Abstract
Pre-harvest sprouting refers to the precocious germination of the grain in the spike prior to harvest as a result of moist weather conditions at harvest time. From the agricultural viewpoint, it is necessary to impose an exogenous dormancy to wheat seeds in order to improve the resistance of seed to pre-harvest sprouting. In this regard, we found that clove bud essential oil is a strong inhibitor for wheat seed germination. The extract obtained from clove bud by supercritical fluid extraction using CO2 as solvent minimized the number of extracts to two compounds, eugenol and eugenyl acetate. Eugenol, as the main constituent of the oil, was responsible for its strong inhibitory activity in wheat seeds. The aqueous solution of clove bud oil was submitted to germination assay at various concentrations from 50 to 400 mg/L. Complete inhibition of seed germination was recorded when the concentration was 400 mg/L. Roots and sprouts have similar sensitivity to inhibitory effect. In an empirical study, the synergistic cooperation of eugenol and eugenyl acetate from clove bud oil in the inhibition of seed germination was found to be a 1:1 ratio. The clove bud essential oil is widely available and will broaden the horizon of applications for natural and safe inhibitors in the fields.
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Acknowledgments
The authors thank the preliminary assistance of Prof. Tadahiro Kato for teaching the principles of germination. The financial support from Ministry of Science, Research and Technology of Iran is gratefully acknowledged. We also thank Mr. Masoum Aghazadeh and Mr. Ghasem Firouzzadeh Pasha for ANOVA calculations.
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Darabi, H.R., Mohandessi, S., Balavar, Y. et al. Clove bud oil: an efficient, economical and widely available oil for the inhibition of wheat seed germination. Environ Chem Lett 9, 519–524 (2011). https://doi.org/10.1007/s10311-011-0312-8
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DOI: https://doi.org/10.1007/s10311-011-0312-8