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Kulturmedien, Kryokonservierung und Co. – relevante Einflussfaktoren im In‑vitro-Fertilisations-Labor

Culture media, cryopreservation and Co.: relevant influencing factors in the in vitro fertilization laboratory

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Gynäkologische Endokrinologie Aims and scope

Zusammenfassung

Die extrakorporale Befruchtung, die Kultur von Embryonen und die Kryokonservierung sind hinsichtlich der Gesundheit der entstehenden Kinder und möglichen Langzeitfolgen trotz mehr als 4 Mio. geborener Kinder weltweit noch nicht vollständig untersucht. Einerseits mangelte es anfänglich an einer vollständigen Erfassung von Patientinnen- und Patientendaten, andererseits sind viele Laborparameter bis heute nur zentrumsintern dokumentiert. Neben Gesundheitsdaten nach einer In-vitro-Fertilisations(IVF)-Behandlung bzw. intrazytoplasmatischen Spermieninjektion (ICSI) gibt es für den Einsatz von kontinuierlichen und sequenziellen Medien („1-step“ und „2-step“) relativ viele Daten zur Gesundheit der Kinder. Bei anderen Methoden, wie der Kryokonservierung mittels „slow freezing“ oder Vitrifikation, liegen häufig nur Geburtsgewicht, Geburtsmodus und Abortrate vor. Eine vollständige Datenerfassung, die neben allen Patientinnen- und Patientenparametern auch die Labordaten berücksichtigt, ist derzeit noch nicht verfügbar.

Abstract

Extracorporal fertilization, embryo culture and cryopreservation have not been fully investigated as yet regarding the health of the resulting children and possible long-term effects, despite the fact that more than 4 million children have been born worldwide. On the one hand, comprehensive recording of patient data was initially lacking, while on the other many laboratory parameters are still only documented internally at the respective centres. In addition to health data after in vitro fertilization (IVF) treatment or intracytoplasmic sperm injection (ICSI), there is a relatively large amount of data on childrenʼs health for the use of continuous and sequential media (“1-step” and “2-step”). With other methods, such as cryopreservation using “slow freezing” or vitrification, often only birth weight, birth mode and miscarriage rate are available. Comprehensive data collection, including all patient parameters as well as laboratory data, is not available yet.

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  1. Frauenklinik, UniKiD, Universitätsklinikum Düsseldorf, Moorenstr. 5, 40225, Düsseldorf, Deutschland

    D. M. Baston-Büst

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Für diesen Beitrag wurden von der Autorin keine Studien an Menschen oder Tieren durchgeführt. Für die aufgeführten Studien gelten die jeweils dort angegebenen ethischen Richtlinien.

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B. Sonntag, Hamburg

G. Griesinger, Lübeck

R. Felberbaum, Kempten

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Baston-Büst, D.M. Kulturmedien, Kryokonservierung und Co. – relevante Einflussfaktoren im In‑vitro-Fertilisations-Labor. Gynäkologische Endokrinologie 18, 199–203 (2020). https://doi.org/10.1007/s10304-020-00337-8

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