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Cytocompatibility of filling pastes by primary teeth root simulating model

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Abstract

Evaluate the cytocompatibility of Calen®/ZO, Calcicur®, Vitapex®, Endoflas®, and zinc oxide/eugenol-based (ZOE) root canal pastes (RCP) to human primary osteoblasts (HPO) through a simplified model for primary teeth. The model employed pipette tips filled with 0.037 g of paste, exposed to 185 µL of culture medium for 24 h (n = 6). Release of components was analysed by Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR). HPO were exposed to conditioned media for 24 h. Cell viability was assessed by cell density and metabolic activity, and release of interleukin 6 (IL-6), vascular endothelial growth factor (VEGF) and fibroblast growth factor (bFGF) by immunological assay. Physicochemical properties and antimicrobial efficacy were also evaluated. 1H-NMR spectra analysis showed similarity between ZOE, Endoflas®, Calcicur®, and Vitapex® compared to Calen®/ZO and positive control, which showed distinct released components. Calen®/ZO and Calcicur® exhibited high alkaline pH in all periods and showed similar solubility. Calen®/ZO, ZOE, and Vitapex® showed similar flow rate. Calen®/ZO, Calcicur®, and Vitapex® did not exhibit antimicrobial efficacy. Calen®/ZO presented cytotoxicity (p < 0.05). Pastes did not increase IL-6 release compared to control. Apart from Vitapex®, all pastes significantly induced VEGF/bFGF release. Interactive effects among released products may affect biological response to filling pastes. Calcicur®, ZOE, Endoflas® and Calen®/ZO presented good to moderate cytocompatibility, with low impact on pro-inflammatory cytokine release and induction of growth factors of interest to tissue repair. This simplified model, specific for the evaluation of the cytocompatibility of RCPs on primary teeth, suggests how these pastes might contribute to bone repair in clinical situations of apical periodontitis in children.

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Acknowledgements

The authors wish to acknowledge SS White Materiais Dentários Ltda. for providing the experimental Calen®/ZO paste and Amanda Souza Nunes Monteiro [TCT FAPERJ], Marlei Gomes da Silva, Adriana Brandão Ribeiro Linhares and Professor Rafael Silva Duarte for the assistance. This work was supported by Fundação de Amparo à Pesquisa do Rio de Janeiro—FAPERJ [Grant Numbers E-26/202-400/2017 and E-26/202-399/2017] and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES—[Finance code 0001].

Funding

This work was supported by Fundação de Amparo à Pesquisa do Rio de Janeiro—FAPERJ [Grant Numbers E-26/202-400/2017, E-26/202-399/2017 and E-26/203.017/2017] and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES—[Finance code 0001], Brazil.

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Authors and Affiliations

  1. Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rua Prof Rodolpho Paulo Rocco, 325, Ilha do Fundão, Rio de Janeiro, 21941-971, Brazil

    Andréa Vaz Braga Pintor, Mariana Coutinho Sancas, Andréa Fonseca-Gonçalves, Liana Bastos Freitas-Fernandes, Ivete Pomarico Ribeiro de Souza & Laura Guimarães Primo

  2. Post-Graduation Program in Science and Biotechnology, Universidade Federal Fluminense, Rua Marquês do Paraná, 303, Niterói, Rio de Janeiro, Brazil

    Luciana Domênico Queiroz, Ana Carolina Batista Brochado & Janaína Spoladore

  3. Department of Community and Preventive Dentistry, Universidade do Estado do Rio de Janeiro, Boulevard 28 de Setembro, 157, Rio de Janeiro, Brazil

    Tatiana Kelly S. Fidalgo

  4. National Center of Nuclear Magnetic Resonance, Medical Biochemistry, Universidade Federal do Rio de Janeiro, Av Carlos Chagas Filho, 373, Rio de Janeiro, Brazil

    Ana Paula Valente

  5. Department of Molecular and Cellular Biology, Biology Institute, Universidade Federal Fluminense, Rua Marquês do Paraná, 303, Niterói, Rio de Janeiro, Brazil

    Gutemberg Gomes Alves

Authors
  1. Andréa Vaz Braga Pintor
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  2. Luciana Domênico Queiroz
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Contributions

LGP, GGA, AFG, TKF, APV and AVBP contributed to the study conception and design. MCS and AVBP conducted the physico-chemical experiments, collected and analysed the data. LDO and AVBP conducted the cytotoxicty assays, collected and analysed the data. ACBB, JS, GGA and AVBP conducted the immunological assays, collected and analysed the data. AFG and AVBP conducted the antimicrobial activity screening, collected and analysed the data. TKF, LBFF, APV and AVBP conducted the 1H-NMR assays, collected and analysed the data. AVBP, TKF and GGA drafted the first version of the manuscript. AVBP, LGP, TKF, LBFF, APV, IPRS and GGA led the writing and edited the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Laura Guimarães Primo.

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Conflict of interest

Author Andréa Vaz Braga Pintor declares that she has no conflict of interest. Author Luciana Domênico Queiroz declares that she has no conflict of interest. Author Mariana Coutinho Sancas declares that she has no conflict of interest. Author Ana Carolina Batista Brochado declares that she has no conflict of interest. Author Janaína Spoladore declares that she has no conflict of interest. Author Andréa Fonseca-Gonçalves declares that she has no conflict of interest. Author Tatiana Kelly Fidalgo declares that she has no conflict of interest. Author Liana Bastos Freitas-Fernandes declares that she has no conflict of interest. Author Ana Paula Valente declares that she has no conflict of interest. Author Ivete Pomarico Ribeiro de Souza declares that she has no conflict of interest. Author Laura Guimarães Primo declares that she has no conflict of interest. Author Gutemberg Gomes Alves declares that he has no conflict of interest.

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All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and national research committees and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Pintor, A.V.B., Queiroz, L.D., Sancas, M.C. et al. Cytocompatibility of filling pastes by primary teeth root simulating model. Odontology 109, 174–183 (2021). https://doi.org/10.1007/s10266-020-00541-9

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