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Effect of adhesive system application for cavities prepared with erbium, chromium: yttrium scandium gallium garnet laser on rat dental pulp tissue

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Abstract

We examined the effects of adhesive systems under study applied for a laser-cut cavity using an Er,Cr:YSGG laser on rat dental pulp at 24 h and 14 days postoperatively. Group 1, laser-cut cavities were treated with a self-etching-primer and bonding agent; group 2, pretreated with a phosphoric-acid, and then treated with a self-etching-primer and bonding agent; group 3, pretreated with a phosphoric-acid and sodium-hypochlorite, and then treated with a self-etching-primer and bonding agent; and group 4, treated with an all-in-one adhesive. A flowable resin composite was used as filling material for each cavity treated with each group. A glass-ionomer-cement was used as a control. The following items were evaluated: pulp-tissue-disorganization (PTD), inflammatory-cell-infiltration (ICI), tertiary-dentin-formation (TDF), and bacterial-penetration (BP). The results were statistically analyzed using the Kruskal–Wallis test and Mann–Whitney U test. No significant differences were observed among the experimental groups for all parameters after 24 h and 14 days (P > 0.05). The majority of the specimens showed PTD with edema formation after 24 h; however, all the specimens demonstrated pulpal healing with TDF after 14 days. On the parameter of TDF, all groups showed significant differences between the two postoperative periods (P < 0.01). On the parameter of ICI, a significant difference was found between the two postoperative periods in group 4 (P < 0.05). No specimens showed BP. The pretreatment on the cavity prepared with the laser using phosphoric-acid or sodium-hypochlorite did not affect the dental pulp healing of rat tooth.

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Acknowledgments

This study was supported in part by Grants-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (No. 23234567). The authors thank Kuraray Noritake Dental and GC Inc. for the experimental adhesive resins and other materials they generously provided. The authors are deeply indebted to Emeritus Prof. S. Yoshie, Department of Histology, The Nippon Dental University School of Life Dentistry at Niigata, for valuable advice and suggestions.

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Authors and Affiliations

  1. Advanced Operative Dentistry, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan

    Mayo Takada & Koichi Shinkai

  2. Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan

    Masaya Suzuki & Koichi Shinkai

  3. Department of Histology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan

    Maiko Haga-Tsujimura

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  1. Mayo Takada
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Correspondence to Koichi Shinkai.

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Kuraray Noritake Dental and GC provided the materials used in this study.

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Takada, M., Suzuki, M., Haga-Tsujimura, M. et al. Effect of adhesive system application for cavities prepared with erbium, chromium: yttrium scandium gallium garnet laser on rat dental pulp tissue. Odontology 105, 300–310 (2017). https://doi.org/10.1007/s10266-016-0278-x

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